Forensic Biology Department, The Center for Forensic Science Research and Education, Willow Grove, Pennsylvania, USA.
Department of Biology, University of Duisburg-Essen, Essen, Germany.
Genet Test Mol Biomarkers. 2021 May;25(5):376-380. doi: 10.1089/gtmb.2020.0341. Epub 2021 Apr 29.
As the demand in genetic testing increases, various fields look toward collection methods that are noninvasive and efficient in recovering deoxyribonucleic acid (DNA) for testing that will allow for high first-pass success rates. Two extraction methods (PrepFiler™ Express Forensic Extraction and the Maxwell RSC Buccal Swab DNA Kits) were optimized to increase DNA yield from a buccal cell collection device (Gentueri's CollectEject™ Swab). Buccal swabs were processed under varying incubation parameters using a forensic workflow. The PrepFiler method was adjusted to test longer incubation times and more aggressive agitation. The Maxwell method was adjusted to test incubation temperatures and duration. Quantitative results showed that increased agitation can yield more DNA through the PrepFiler extraction, but longer incubation times did not increase DNA recovery. The results from the Maxwell study showed no significant difference between incubation temperatures or times. The results indicate that various applied genetic fields can utilize a noninvasive, simple collection method using the CollectEject device in conjunction with extraction methods already implemented in laboratories to collect 5000 ng of DNA or greater from a buccal cell collection.
随着基因检测需求的增加，各个领域都在寻求非侵入性且高效的方法来提取脱氧核糖核酸 (DNA) 进行检测，以提高首次通过率。本文对两种提取方法（PrepFiler Express 法医提取试剂盒和 Maxwell RSC 口腔拭子 DNA 试剂盒）进行了优化，以增加从口腔细胞采集装置（Gentueri 的 CollectEject 拭子）中提取的 DNA 产量。使用法医工作流程，在不同的孵育参数下对口腔拭子进行处理。PrepFiler 方法被调整以测试更长的孵育时间和更剧烈的搅拌。Maxwell 方法被调整以测试孵育温度和时间。定量结果表明，PrepFiler 提取法通过增加搅拌可以获得更多的 DNA，但更长的孵育时间并不能增加 DNA 回收率。Maxwell 研究的结果表明，孵育温度或时间之间没有显著差异。结果表明，各个应用遗传学领域可以利用非侵入性、简单的采集方法，结合实验室中已经实施的提取方法，从口腔细胞采集装置中收集 5000ng 或更多的 DNA。
