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鼠李糖脂对幼虫的作用:对抗抗性选择的潜在武器。

Rhamnolipids on larvae: a potential weapon against resistance selection.

作者信息

de Paula Siqueira Tatiane, Barbosa Wagner Faria, Rodrigues Edmo Montes, Miranda Franciane Rosa, de Souza Freitas Fernanda, Martins Gustavo Ferreira, Tótola Marcos Rogério

机构信息

Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente, Departamento de Microbiologia, Universidade Federal de Viçosa, Av. P.H. Rolfs s/n, Centro, Viçosa, Minas Gerais Brazil.

Programa de Pós-Graduação em Entomologia, Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais Brazil.

出版信息

3 Biotech. 2021 Apr;11(4):172. doi: 10.1007/s13205-021-02716-9. Epub 2021 Mar 18.

Abstract

The resistance of to chemical insecticides has been reported and our work proposes the use of biosurfactants as an alternative larvicide. We evaluated the effect of rhamnolipids against larvae of pyrethroid-resistant and susceptible strains. Time-mortality and sublethal effects were evaluated via survival analysis and swimming behavior, respectively. Rhamnolipids showed larvicidal effect at all tested concentrations. Rhamnolipids at 300 mg L killed 100% of both susceptible and resistant larvae within 24 h of exposure and 99% after 30-days stored (pyrethroid-susceptible larvae). Regarding the sublethal effects, the swimming rate was reduced in 50 and 100 mg L of rhamnolipids in grouped (pyrethroid-susceptible) larvae. Rhamnolipids at 50 mg L reduced the distance and speed and increased the number of stops and resting time of individualized pyrethroid-susceptible larvae. The larvicidal effect of the rhamnolipids evaluated demonstrates that these compounds represent an alternative to control .

摘要

已报道了对化学杀虫剂的抗性,我们的研究提出使用生物表面活性剂作为替代杀幼虫剂。我们评估了鼠李糖脂对拟除虫菊酯抗性和敏感品系幼虫的影响。分别通过生存分析和游泳行为评估了时间-死亡率和亚致死效应。鼠李糖脂在所有测试浓度下均显示出杀幼虫效果。300 mg/L的鼠李糖脂在暴露24小时内杀死了100%的敏感和抗性幼虫,对于储存30天的拟除虫菊酯敏感幼虫,死亡率为99%。关于亚致死效应,在分组的(拟除虫菊酯敏感)幼虫中,50和100 mg/L的鼠李糖脂降低了游泳速率。50 mg/L的鼠李糖脂降低了单个拟除虫菊酯敏感幼虫的游动距离和速度,并增加了停顿次数和静止时间。所评估的鼠李糖脂的杀幼虫效果表明,这些化合物是控制……的一种替代方法。 (原文中“control ”后缺少具体对象)

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