比较改良碳青霉烯酶灭活法检测肠杆菌科中圭亚那扩展谱β-内酰胺酶型碳青霉烯酶的表型。

Comparative Evaluation of the Modified Carbapenem Inactivation Method for Phenotypic Detection of Guiana Extended-Spectrum β-Lactamase-Type Carbapenemases in Enterobacterales.

机构信息

Department of Laboratory Medicine, Daegu Catholic University School of Medicine, Daegu, South Korea.

出版信息

Lab Med. 2021 Nov 2;52(6):578-583. doi: 10.1093/labmed/lmab026.

DOI:10.1093/labmed/lmab026

PMID:33928355

Abstract

OBJECTIVE

We comparatively evaluated the performance of 3 phenotypic tests for the detection of carbapenemase production.

MATERIALS AND METHODS

Carbapenemase production was evaluated using the modified Hodge test (MHT), the modified carbapenemase inhibition method (mCIM), and the Rapidec Carba NP test (RCNP).

RESULTS

Among the 170 isolates, 79 were CP-CRE and 91 were non-CP-CRE. The CP-CRE isolates produced GES-5 (n = 66), KPC (n = 4), NDM (n = 7), NDM and OXA-48 (n = 1), and VIM (n = 1). For KPC producers, all 3 methods showed a sensitivity of 75%. The sensitivities of MHT, mCIM, and RCNP were 14.3%, 100%, and 71.4%, respectively, for NDM producers, and 1.5%, 12.1%, and 18.2% for GES-5 producers, respectively.

CONCLUSION

The performance of the phenotypic tests varied depending on the type of carbapenemase. For intensive infection control, phenotypic and molecular tests are required for the detection of common and rare types of carbapenemases.

摘要

目的

我们比较评估了 3 种表型检测方法用于检测产碳青霉烯酶的性能。

材料和方法

使用改良 Hodge 试验（MHT）、改良碳青霉烯酶抑制法（mCIM）和 Rapidec Carba NP 试验（RCNP）评估产碳青霉烯酶。

结果

在 170 株分离株中，79 株为 CP-CRE，91 株为非 CP-CRE。CP-CRE 分离株产生 GES-5（n=66）、KPC（n=4）、NDM（n=7）、NDM 和 OXA-48（n=1）和 VIM（n=1）。对于 KPC 产生菌，3 种方法的敏感性均为 75%。MHT、mCIM 和 RCNP 对 NDM 产生菌的敏感性分别为 14.3%、100%和 71.4%，对 GES-5 产生菌的敏感性分别为 1.5%、12.1%和 18.2%。