Chronic Immune Reactions, German Rheumatism Research Center, A Leibniz Institute, Berlin, Germany.
Institute of Immunology, University Hospital Schleswig-Holstein, Kiel, Germany.
Methods Mol Biol. 2021;2285:91-98. doi: 10.1007/978-1-0716-1311-5_7.
Under chronic inflammatory conditions, T and B cells are frequently found in nonlymphoid tissues. We recently identified a follicular helper-like T cell population in inflamed lung tissue, which drives the local differentiation of antigen-specific B cells into germinal center-like cells and plasma blasts. Here, we describe a lung inflammation mouse model, which is ideally suited to analyze antigen-specific T and B cells in secondary lymphoid organs and inflamed nonlymphoid tissue in parallel.
在慢性炎症情况下，T 细胞和 B 细胞经常在非淋巴组织中被发现。我们最近在发炎的肺组织中鉴定出一种滤泡辅助样 T 细胞群，它驱动抗原特异性 B 细胞在局部分化为生发中心样细胞和浆母细胞。在这里，我们描述了一种肺部炎症小鼠模型，该模型非常适合平行分析次级淋巴器官和炎症非淋巴组织中的抗原特异性 T 细胞和 B 细胞。
