Chronic Immune Reactions, German Rheumatism Research Centre, A Leibniz Institute, Berlin, Germany.
Institute of Immunology, University Hospital Schleswig-Holstein, Kiel, Germany.
Methods Mol Biol. 2021;2285:77-90. doi: 10.1007/978-1-0716-1311-5_6.
Follicular helper T (Tfh) cells play a key role in B cell activation and differentiation. Within recent years, distinct subsets of follicular T cells, including regulatory and cytotoxic T cells, have been identified. Apart from classical Tfh cells in secondary lymphoid organs, Tfh-like cells are found in chronically inflamed nonlymphoid tissues. Here, we provide protocols to identify different follicular T cell subsets in murine and human tissues by flow cytometry. This chapter also contains an immunization protocol for the induction of large numbers of Tfh cells in mice.
滤泡辅助 T（Tfh）细胞在 B 细胞的激活和分化中起着关键作用。近年来，已鉴定出滤泡 T 细胞的不同亚群，包括调节性 T 细胞和细胞毒性 T 细胞。除了次级淋巴器官中的经典 Tfh 细胞外，Tfh 样细胞还存在于慢性炎症的非淋巴组织中。在这里，我们提供了通过流式细胞术鉴定小鼠和人组织中不同滤泡 T 细胞亚群的方案。本章还包含了在小鼠中诱导大量 Tfh 细胞的免疫方案。
