长链非编码 RNA PRNCR1 通过调控 miR-326/FSCN1 轴促进口腔鳞状细胞癌的恶性进展。
LncRNA PRNCR1 aggravates the malignancy of oral squamous cell carcinoma by regulating miR-326/FSCN1 axis.
机构信息
Department of Stomatology, Yantaishan Hospital, Yantai, Shandong Province, China.
出版信息
Eur Rev Med Pharmacol Sci. 2021 Apr;25(8):3226-3234. doi: 10.26355/eurrev_202104_25731.
OBJECTIVE
The important regulatory mechanism of lncRNA PRNCR1 has been emphasized in malignant tumors. However, the role of lncRNA PRNCR1 remains unclear in oral squamous cell carcinoma (OSCC). The purpose of this study is to reveal the role of lncRNA PRNCR1 in OSCC.
PATIENTS AND METHODS
RT-qPCR was used to detect mRNA expression. The functional mechanism of lncRNA PRNCR1 in OSCC was investigated by CCK-8, transwell and Luciferase reporter assays.
RESULTS
LncRNA PRNCR1 was upregulated in OSCC and promoted cell proliferation, migration and invasion. LncRNA PRNCR1 directly binds to miR-326. The mutual inhibition between the expressions of lncRNA PRNCR1 and miR-326 was identified in OSCC. In addition, miR-326 restrained cell proliferation, migration and invasion in OSCC. Further, miR-326 directly targets FSCN1. FSCN1 expression was positively regulated by lncRNA PRNCR1 in OSCC. And FSCN1 promoted the progression of OSCC and aggravated the carcinogenic effect of lncRNA PRNCR1 in OSCC.
CONCLUSIONS
LncRNA PRNCR1 promotes the progression of OSCC by functioning as a miR-326 'sponge' to upregulate FSCN1 expression.
目的
长链非编码 RNA PRNCR1 的重要调控机制在恶性肿瘤中已得到强调。然而，lncRNA PRNCR1 在口腔鳞状细胞癌（OSCC）中的作用尚不清楚。本研究旨在揭示 lncRNA PRNCR1 在 OSCC 中的作用。
患者与方法
采用 RT-qPCR 检测 mRNA 表达。通过 CCK-8、Transwell 和荧光素酶报告基因检测实验研究 lncRNA PRNCR1 在 OSCC 中的功能机制。
结果
lncRNA PRNCR1 在 OSCC 中上调，并促进细胞增殖、迁移和侵袭。lncRNA PRNCR1 可直接与 miR-326 结合。在 OSCC 中鉴定出 lncRNA PRNCR1 和 miR-326 表达之间的相互抑制关系。此外，miR-326 抑制 OSCC 中的细胞增殖、迁移和侵袭。进一步研究表明，miR-326 可直接靶向 FSCN1。lncRNA PRNCR1 在 OSCC 中正向调控 FSCN1 的表达。并且 FSCN1 促进 OSCC 的进展，并加重 lncRNA PRNCR1 在 OSCC 中的致癌作用。
结论
lncRNA PRNCR1 通过作为 miR-326 的“海绵”来上调 FSCN1 表达，从而促进 OSCC 的进展。
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