长链非编码 RNA CRNDE 通过 miR-423-5p/FSCN1 轴促进卵巢癌细胞的增殖、迁移和侵袭。

LncRNA CRNDE promotes cell proliferation, migration and invasion of ovarian cancer via miR-423-5p/FSCN1 axis.

机构信息

Department of Pathology, the First Medical Center of PLA General Hospital, Beijing, 100853, China.

Institute of Oncology, the Fifth Medical Center of PLA General Hospital, Beijing, 100853, China.

出版信息

Mol Cell Biochem. 2022 May;477(5):1477-1488. doi: 10.1007/s11010-022-04382-8. Epub 2022 Feb 15.

DOI:10.1007/s11010-022-04382-8

PMID:35166986

Abstract

Ovarian cancer seriously threatens the health of women. LncRNA CRNDE is known to be upregulated in ovarian cancer. However, the mechanism by which CRNDE regulates the progress of ovarian cancer is largely unknown. MTT assay was applied to measure the cell viability. Colony formation assay was used to measure the cell proliferation. Cell migration was tested by wound healing, and Transwell assay was performed to detect cell invasion. In addition, the expression of miR-423-5p, CRNDE and FSCN1 were detected by RT-qPCR and western blotting, respectively. Meanwhile, dual-luciferase reporter assay and RIP assay were performed to explore the correlation between miR-423-5p and CRNDE (or FSCN1). CRNDE and FSCN1 were upregulated in ovarian cancer cells (SKOV3, CAOV-3, IGROV1, A2780 and C13K), while miR-423-5p was downregulated. Moreover, silencing of FSCN1/CRNDE significantly decreased proliferation, migration and invasion of ovarian cancer cells (SKOV3 and CI3K) via suppressing MMP-2 and MMP-9. In addition, CRNDE could sponge miR-423-5p, and FSCN1 was confirmed to be the direct target of miR-423-5p. Furthermore, CRNDE knockdown-induced inhibition of FSCN1 was notably reversed by miR-423-5p downregulation. Knockdown of CRNDE inhibited cell proliferation, migration and invasion of ovarian cancer via miR-423-5p/FSCN1 axis. Thus, CRNDE may serve a new target for ovarian cancer.

摘要

卵巢癌严重威胁着女性的健康。已知长链非编码 RNA CRNDE 在卵巢癌中上调。然而，CRNDE 调节卵巢癌进展的机制在很大程度上尚不清楚。MTT 法用于测量细胞活力。集落形成实验用于测量细胞增殖。通过划痕愈合试验检测细胞迁移，通过 Transwell 试验检测细胞侵袭。此外，通过 RT-qPCR 和 Western blot 分别检测 miR-423-5p、CRNDE 和 FSCN1 的表达。同时，进行双荧光素酶报告基因实验和 RIP 实验来探索 miR-423-5p 与 CRNDE（或 FSCN1）之间的相关性。CRNDE 和 FSCN1 在卵巢癌细胞（SKOV3、CAOV-3、IGROV1、A2780 和 C13K）中上调，而 miR-423-5p 下调。此外，沉默 FSCN1/CRNDE 通过抑制 MMP-2 和 MMP-9 显著降低卵巢癌细胞（SKOV3 和 CI3K）的增殖、迁移和侵袭。此外，CRNDE 可以海绵 miR-423-5p，FSCN1 被证实是 miR-423-5p 的直接靶标。此外，CRNDE 敲低诱导的 FSCN1 抑制作用通过 miR-423-5p 下调显著逆转。CRNDE 敲低通过 miR-423-5p/FSCN1 轴抑制卵巢癌细胞的增殖、迁移和侵袭。因此，CRNDE 可能成为卵巢癌的新靶点。