The Centre for Drug Research and Development, Guangdong Pharmaceutical University, Guangzhou, Guangdong, P. R. China.
Department of Immunology, School of Life Sciences and Biopharmaceuticals Engineering, Guangdong Pharmaceutical University, Guangzhou, Guangdong, P. R. China.
Phytochem Anal. 2021 Nov;32(6):1082-1101. doi: 10.1002/pca.3050. Epub 2021 Apr 30.
Polygoni Multiflori Caulis (PMC) has been used as a traditional Chinese medicine for a long time in China. However, hepatotoxic events of PMC have been reported in recent years, but the potential toxic compounds have remained unclear. Dianthrones as the secondary plant metabolites were revealed to potential hepatotoxicity in a previous study. However, no reports focused on dianthrones in PMC.
In the quest for exploring potential hepatotoxic compounds in PMC, the aim of this work was to undertake a comprehensive characterisation of dianthrones in PMC.
A simple and effective macroporous absorbent resin column chromatography method was established in this study to enrich the minor dianthrones from PMC extracts. Exploration and characterisation of dianthrones in PMC was conducted by an ultra-high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) method and information dependent acquisition (IDA) mode. The aglycones of dianthrone glycosides were further verified by acid hydrolysis method.
Seventy-two dianthrone glycosides and their five aglycones were discovered and tentatively characterised in PMC for the first time, of which 29 dianthrones were identified as potential new compounds. Dianthrone glycosides could be classified into three types according to their aglycone structures, and their fragmentation pathway rules and diagnosed ions were also summarised comprehensively.
This was the first comprehensive investigation on dianthrones in PMC. The result would help to fully understand the phytochemical constituents and toxic components in PMC, and highlight the need for further toxicological investigations of the dianthrones in PMC due to their potential hepatotoxicity correlation.
在中国，何首乌（PMC）作为一种传统中药已经使用了很长时间。然而，近年来已有关于 PMC 肝毒性事件的报道，但潜在的毒性化合物仍不清楚。在之前的研究中，二蒽酮类化合物被揭示为潜在的肝毒性物质。然而，目前尚无关于 PMC 中二蒽酮类化合物的报道。
在探索 PMC 中潜在肝毒性化合物的过程中，本研究旨在全面分析 PMC 中二蒽酮类化合物。
本研究建立了一种简单有效的大孔吸附树脂柱色谱法，从 PMC 提取物中富集微量二蒽酮类化合物。采用超高效液相色谱-四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）法和信息依赖性采集（IDA）模式，对 PMC 中二蒽酮类化合物进行了探索和表征。二蒽酮糖苷的苷元通过酸水解法进一步验证。
首次在 PMC 中发现并初步鉴定了 72 种二蒽酮糖苷及其 5 种苷元，其中 29 种二蒽酮被鉴定为潜在的新化合物。根据苷元结构，二蒽酮糖苷可分为三类，并对其裂解途径规律和诊断离子进行了全面总结。
这是首次对 PMC 中二蒽酮类化合物的全面研究。研究结果有助于全面了解 PMC 中的植物化学成分和毒性成分，并强调由于其潜在的肝毒性相关性，需要进一步对 PMC 中二蒽酮类化合物进行毒理学研究。
