Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, Guangzhou, P. R. China.
Jiangsu Kanion Pharmaceutical Co. Ltd., Lianyungang, P. R. China.
J Sep Sci. 2017 May;40(10):2107-2116. doi: 10.1002/jssc.201601255. Epub 2017 Apr 18.
Polygoni Multiflori Caulis is a traditional Chinese medicine that has been used for a long time to treat sleep disorders. However, the multiple chemical composition analysis has not been reported. In this study, a simple, rapid and effective ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry method was established to characterize the components of Polygoni Multiflori Caulis. In addition, a chemical comparative analysis was performed with Polygoni Multiflori Radix, another traditional Chinese medicine from the same plant, through multivariate statistical analysis and semi-quantitative analysis to screen the difference in chemical ingredients between these two herbal medicines from same medicinal plant. A total of 33 peaks were detected within 25 min, and 28 of them were identified or tentatively characterized by comparing the retention time and mass spectrometry data. Based on the results, 12 characteristic components were screened out by multivariate statistical analysis, and their content change trends were compared by semi-quantitative analysis.
何首乌是一种传统中药，长期以来一直被用于治疗睡眠障碍。然而，其多种化学成分的分析尚未见报道。本研究建立了一种简单、快速、有效的超高效液相色谱-四极杆飞行时间质谱联用方法，用于表征何首乌的成分。此外，通过多变量统计分析和半定量分析，对同属植物的另一种中药制何首乌进行了化学比较分析，以筛选这两种来自同一药用植物的草药之间化学成分的差异。在 25 分钟内检测到 33 个峰，其中 28 个通过保留时间和质谱数据进行比较进行了鉴定或初步表征。基于这些结果，通过多变量统计分析筛选出 12 个特征成分，并通过半定量分析比较其含量变化趋势。
