IITB-Monash Research Academy, Mumbai, India.
Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, India.
Elife. 2021 Apr 30;10:e62524. doi: 10.7554/eLife.62524.
We demonstrate a technique for investigating the energetics of flagella or cilia. We record the planar beating of tethered mouse sperm at high resolution. Beating waveforms are reconstructed using proper orthogonal decomposition of the centerline tangent-angle profiles. Energy conservation is employed to obtain the mechanical power exerted by the dynein motors from the observed kinematics. A large proportion of the mechanical power exerted by the dynein motors is dissipated internally by the motors themselves. There could also be significant dissipation within the passive structures of the flagellum. The total internal dissipation is considerably greater than the hydrodynamic dissipation in the aqueous medium outside. The net power input from the dynein motors in sperm from -knockout mice is significantly smaller than in wildtype samples, indicating that ion-channel regulation by cysteine-rich secretory proteins controls energy flows powering the axoneme.
我们展示了一种研究鞭毛或纤毛能量学的技术。我们以高分辨率记录了束缚的小鼠精子的平面摆动。通过对中轴线切向角轮廓的本征正交分解来重建摆动波形。利用能量守恒定律，从观察到的运动学中获得由动力蛋白马达施加的机械功率。动力蛋白马达施加的大部分机械功率被马达本身内部耗散。鞭毛的被动结构内也可能存在显著的耗散。总的内部耗散明显大于水相介质中的流体动力耗散。来自 - 敲除小鼠精子的动力蛋白马达的净功率输入明显小于野生型样本，这表明富含半胱氨酸的分泌蛋白对离子通道的调节控制着为轴丝提供动力的能量流。
