Opt Lett. 2021 May 1;46(9):2176-2179. doi: 10.1364/OL.418240.
Photoswitchable fluorescence is a powerful technique to realize super-resolution imaging, highlighting, and optical storage, while its multiplexing capability is limited. Raman scattering is attracting attention because it generates narrowband vibrational signatures, which are potentially useful for highly multiplexed detection of different constituents. Here, we demonstrate photoswitchable stimulated Raman scattering (SRS) spectroscopy and microscopy where narrowband vibrational signatures are switched with full reversibility at high speed. The demonstration of live-cell photoswitchable SRS imaging shows good sensitivity and compatibility with biological living systems.
光致变色荧光是一种实现超分辨率成像、高亮显示和光存储的强大技术，但其多路复用能力有限。拉曼散射由于产生窄带振动特征而受到关注，这些特征可能对不同成分的高度多路复用检测非常有用。在这里，我们展示了光致变色受激拉曼散射（SRS）光谱学和显微镜技术，其中窄带振动特征可以在高速下完全可逆地切换。活细胞光致变色 SRS 成像的演示显示了良好的灵敏度和与生物活系统的兼容性。
