Wang Haomin, Lee Dongkwan, Wei Lu
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
Chem Biomed Imaging. 2023 Mar 28;1(1):3-17. doi: 10.1021/cbmi.3c00004. eCollection 2023 Apr 24.
Chemical imaging based on vibrational contrasts can extract molecular information entangled in complex biological systems. To this end, nonlinear Raman scattering microscopy, mid-infrared photothermal (MIP) microscopy, and atomic force microscopy (AFM)-based force-detected photothermal microscopies are emerging with better chemical sensitivity, molecular specificity, and spatial resolution than conventional vibrational methods. Their utilization in bioimaging applications has provided biological knowledge in unprecedented detail. This Perspective outlines key methodological developments, bioimaging applications, and recent technical innovations of the three techniques. Representative biological demonstrations are also highlighted to exemplify the unique advantages of obtaining vibrational contrasts. With years of effort, these three methods compose an expanding vibrational bioimaging toolbox to tackle specific bioimaging needs, benefiting many biological investigations with rich information in both label-free and labeling manners. Each technique will be discussed and compared in the outlook, leading to possible future directions to accommodate growing needs in vibrational bioimaging.
基于振动对比度的化学成像能够提取复杂生物系统中纠缠的分子信息。为此，非线性拉曼散射显微镜、中红外光热（MIP）显微镜以及基于原子力显微镜（AFM）的力检测光热显微镜正在兴起，它们比传统振动方法具有更高的化学灵敏度、分子特异性和空间分辨率。它们在生物成像应用中的使用以前所未有的细节提供了生物学知识。本视角概述了这三种技术的关键方法学发展、生物成像应用以及近期技术创新。还突出了代表性的生物学实例，以例证获得振动对比度的独特优势。经过多年努力，这三种方法构成了一个不断扩展的振动生物成像工具箱，以满足特定的生物成像需求，通过无标记和标记方式为许多生物学研究提供丰富信息。展望中将对每种技术进行讨论和比较，从而得出适应振动生物成像不断增长需求的可能未来方向。
