Department of Chemistry, Faculty of Arts and Sciences, Kocaeli University, 41380, Kocaeli, Turkey.
Department of Chemistry, Faculty of Arts and Sciences, Bursa Uludag University, 16059, Bursa, Turkey.
Eur Biophys J. 2021 Jul;50(5):771-785. doi: 10.1007/s00249-021-01525-5. Epub 2021 Apr 30.
A novel water soluble ternary copper(II) complex,-Cu(phen)(3-IAA)(HO)·HO-(phen: 1,10-phenanthroline, 3-IAA: 3-indoleacetic acid), has been synthesized and characterized by elemental CHN analysis, ESI-TOF, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with calf thymus DNA (CT-DNA) has been investigated by absorption spectral titration, ethidium bromide (EB) and Hoechst 33258 displacement assay. The interactions between the complex and bovine serum albumin (BSA) were investigated by electronic absorption and fluorescence spectroscopy methods. The experimental results indicate that the fluorescence quenching mechanism between the complex and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (ΔG, ΔH, ΔS) of BSA + complex systems were determined at different temperatures. The binding distance between the complex and BSA was calculated according to Förster non-radiation energy transfer theory (FRET). The effect of the complex on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Furthermore, the oxygen radical scavenging activity of the complex was determined in terms of IC, using the DPPH and HO method, to show that it particularly enables electron loss from radical species. This study highlights the importance of indole and moieties in the development of antioxidant agents. A potent drug candidate novel water soluble ternary copper(II) complex,-[Cu(phen)(3-IAA)(HO)] (ClO)·HO-(phen: 1,10-phenanthroline, 3-IAA: 3-indoleacetic acid), has been synthesized and characterized by elemental CHN analysis, FTIR, ESI-MS and single-crystal X-ray diffraction techniques. The complex has been tested for in vitro biomacromolecular interactions by spectroscopic methods. Furthermore, radical scavenging activities of the complex were also investigated.
一种新型水溶性三元铜（II）配合物-[Cu(phen)(3-IAA)(HO)]（ClO）·HO-（phen：1,10-邻菲啰啉，3-IAA：3-吲哚乙酸）已通过元素 CHN 分析、ESI-TOF、FTIR 和单晶 X 射线衍射技术进行了合成和表征。通过光谱法研究了该配合物与小牛胸腺 DNA（CT-DNA）的体外生物大分子相互作用。还研究了配合物与牛血清白蛋白（BSA）的相互作用，采用电子吸收和荧光光谱法。实验结果表明，配合物与 BSA 之间的荧光猝灭机制是静态猝灭过程。在不同温度下确定了 BSA+配合物体系的 Stern-Volmer 常数、结合常数、结合位点数和相应的热力学参数（ΔG、ΔH、ΔS）。根据福斯特非辐射能量转移理论（FRET）计算了配合物与 BSA 之间的结合距离。还使用同步、二维（2D）和三维（3D）荧光光谱法研究了配合物对 BSA 构象的影响。此外，还通过 DPPH 和 HO 法测定了配合物对氧自由基的清除活性，以表明其特别能够使自由基失去电子。这项研究强调了吲哚和 moieties 在抗氧化剂开发中的重要性。一种新型水溶性三元铜（II）配合物-[Cu(phen)(3-IAA)(HO)]（ClO）·HO-（phen：1,10-邻菲啰啉，3-IAA：3-吲哚乙酸）已被合成并通过元素 CHN 分析、FTIR、ESI-MS 和单晶 X 射线衍射技术进行了表征。通过光谱法研究了该配合物与生物大分子的体外相互作用。此外，还研究了配合物的自由基清除活性。
