Department of Chemistry, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia.
Biometals. 2022 Dec;35(6):1199-1223. doi: 10.1007/s10534-022-00437-x. Epub 2022 Sep 8.
A new ligand 1,2,4-triazino[5,6-b]indol-3-ylimino methyl naphthalene-2-ol (HL) was derived from 5H-[1,2,4]triazino[5,6-b]indol-3-amine and 2-hydroxy-1-naphthaldehyde. The metal complexes of the type [Ni(L)(Bipy)]1/2SO (1), [Cu(L)(Bipy)(HO)]1/2SO (2), [Ni(L)(Phen)]1/2SO (3) and [Cu(L)(Phen)(HO)]1/2SO (4) were synthesized. The ligand (HL) and complexes 1-4 were thoroughly characterized by elemental analysis and spectroscopic methods (FT-IR, ToF-MS, H NMR, C NMR), molar conductance and magnetic moment determination. The Ni(II) complexes 1 and 3 adopt the square planar geometry and Cu(II) complexes 2 and 4 acquire distorted octahedral arrangement. In vitro DNA binding behavior of ligand (HL) and metal complexes 1-4 was explored by fluorescence spectral and ethidium bromide studies. The outcomes reveal that the complexes interact with DNA via non-covalent groove binding and electrostatic interactions. The higher binding constant (K) values of 4.35 × 10 and 9.12 × 10 M for complexes 2 and 4 indicate stronger binding ability with DNA. Moreover, in vitro human serum albumin (HSA) binding experiment with HL and complexes 1-4 reveals conformational modulations in the Trp-214 microenvironments in the subdomain IIA pocket.
一种新的配体 1,2,4-三嗪并[5,6-b]吲哚-3-基亚氨基甲基萘-2-醇（HL）是由 5H-[1,2,4]三嗪并[5,6-b]吲哚-3-胺和 2-羟基-1-萘醛衍生而来。合成了[Ni(L)(Bipy)]1/2SO（1）、[Cu(L)(Bipy)(HO)]1/2SO（2）、[Ni(L)(Phen)]1/2SO（3）和[Cu(L)(Phen)(HO)]1/2SO（4）等类型的金属配合物。配体（HL）和配合物 1-4 通过元素分析和光谱方法（FT-IR、ToF-MS、H NMR、C NMR）、摩尔电导率和磁矩测定进行了彻底的表征。Ni(II)配合物 1 和 3 采用平面四方几何构型，Cu(II)配合物 2 和 4 采用扭曲八面体排列。通过荧光光谱和溴化乙锭研究探讨了配体（HL）和金属配合物 1-4 与 DNA 的体外结合行为。结果表明，配合物通过非共价沟结合和静电相互作用与 DNA 相互作用。配合物 2 和 4 的较高结合常数（K）值 4.35×10 和 9.12×10 M 表明它们与 DNA 具有更强的结合能力。此外，HL 和配合物 1-4 与人类血清白蛋白（HSA）的体外结合实验表明，在亚结构域 IIA 口袋中的 Trp-214 微环境中发生了构象调制。
