Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA, 30332, USA.
Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
Adv Healthc Mater. 2021 Aug;10(15):e2100016. doi: 10.1002/adhm.202100016. Epub 2021 Apr 30.
Recent developments in mesenchymal stromal cell (MSC) therapies have increased the demand for tools to improve their manufacture, including the selection of optimal culture substrate materials. While many clinical manufacturers use planar tissue culture plastic (TCP) surfaces for MSC production, others have begun exploring the use of alternative culture substrates that present a variety of spatial, mechanical, and biochemical cues that influence cell expansion and resulting cell quality. In this review, the effects of culture and material properties distinct from traditional planar TCP surfaces on MSC proliferation, surface marker expression, and commonly used indications for therapeutic potency are examined. The different properties summarized include the use of alternative culture formats such as cellular aggregates or 3D scaffolds, as well as the effects of culture substrate stiffness and presentation of specific adhesive ligands and topographical cues. Specific substrate properties can be related to greater cell expansion and improvement in specific therapeutic functionalities, demonstrating the utility of culture materials in further improving the clinical-scale manufacture of highly secretory MSC products.
间充质基质细胞 (MSC) 治疗的最新进展增加了对改进其制造的工具的需求，包括选择最佳的培养基底材料。虽然许多临床制造商使用平面组织培养塑料 (TCP) 表面来生产 MSC，但其他人已经开始探索使用替代的培养基底，这些基底提供了各种空间、机械和生化线索，影响细胞的扩增和最终细胞质量。在这篇综述中，研究了不同于传统平面 TCP 表面的培养和材料特性对 MSC 增殖、表面标志物表达以及治疗效力的常见适应症的影响。总结的不同特性包括使用替代的培养形式，如细胞聚集体或 3D 支架，以及培养基底硬度和特定粘附配体和形貌线索的影响。特定的基底特性可以与更大的细胞扩增和特定治疗功能的改善相关，证明了培养材料在进一步提高高分泌 MSC 产品的临床规模制造中的实用性。
