Pontificia Universidad Católica del Perú, Departamento de Ciencias - Química, CERMN, Av. Universitaria 1801, Lima 32, Peru.
Pontificia Universidad Católica del Perú, Departamento de Ciencias - Química, CERMN, Av. Universitaria 1801, Lima 32, Peru.
Food Chem. 2021 Oct 1;358:129365. doi: 10.1016/j.foodchem.2021.129365. Epub 2021 Feb 23.
The fruit of Vanilla planifolia is broadly preferred by the agroindustry and gourmet markets due to its refined flavor and aroma. Peruvian Vanilla has been proposed as a possible source for genetic improvement of existing Vanilla cultivars, but, little has been done to facilitate comprehensive studies of these and other Vanilla. Here, a nuclear magnetic resonance (NMR) metabolomic platform was developed to profile for the first time the leaves - organ known to accumulate vanillin putative precursors - of V. planifolia and those of Peruvian V. pompona, V. palmarum, and V. ribeiroi, with the aim to determine metabolic differences among them. Analysis of the NMR spectra allowed the identification of thirty-six metabolites, twenty-five of which were quantified. One-way ANOVA and post-hoc Tukey test revealed that these metabolites changed significantly among species, whilst multivariate-analyses allowed the identification of malic and homocitric acids, together with two vanillin precursors, as relevant metabolic markers for species differentiation.
由于其精致的风味和香气，香草 planifolia 的果实广受农业产业和美食市场的青睐。秘鲁香草已被提议作为现有香草品种遗传改良的可能来源，但对于这些和其他香草的综合研究却鲜有开展。在这里，开发了一种核磁共振（NMR）代谢组学平台，首次对叶片进行了分析 - 已知积累香草醛假定前体的器官 - 以及秘鲁香草、V. palmarum 和 V. ribeiroi 的叶片，旨在确定它们之间的代谢差异。对 NMR 光谱的分析允许鉴定出三十六个代谢物，其中二十五个被定量。单因素方差分析和事后 Tukey 检验表明，这些代谢物在物种间存在显著差异，而多元分析允许鉴定出苹果酸和同型柠檬酸，以及两种香草醛前体，作为物种分化的相关代谢标志物。
