Department of Applied Physics and the Brojde Center for Innovative Engineering and Computer Science, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.
Department of Applied Physics and the Brojde Center for Innovative Engineering and Computer Science, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.
Biosens Bioelectron. 2021 Aug 1;185:113253. doi: 10.1016/j.bios.2021.113253. Epub 2021 Apr 15.
We describe a miniaturized field-deployable biosensor module, designed to function as an element in a sensor network for standoff monitoring and mapping of environmental hazards. The module harbors live bacterial sensor cells, genetically engineered to emit a bioluminescent signal in the presence of preselected target materials, which act as its core sensing elements. The module, which detects and processes the biological signal, composes a digital record that describes its findings, and can be transmitted to a remote receiver. The module is an autonomous self-contained unit that can function either as a standalone sensor, or as a node in a sensor network. The biosensor module can potentially be used for detecting any target material to which the sensor cells were engineered to respond. The module described herein was constructed to detect the presence of buried landmines underneath its footprint. The demonstrated detection sensitivity was 0.25 mg 2,4-dinitrotoluene per Kg soil.
我们描述了一种小型化的现场可部署生物传感器模块，旨在作为传感器网络中的一个元件，用于进行环境危害的远程监测和绘图。该模块包含活的细菌传感器细胞，经过基因工程改造，在存在预先选定的目标材料时会发出生物发光信号，这些目标材料是其核心感测元件。该模块可以检测和处理生物信号，构成描述其发现的数字记录，并可以传输到远程接收器。该模块是一个自主式的独立单元，既可以作为独立的传感器，也可以作为传感器网络中的一个节点。该生物传感器模块可以潜在地用于检测任何目标材料，只要这些目标材料是传感器细胞被设计用来响应的。本文描述的模块是为了检测其足迹下埋地雷的存在而构建的。所展示的检测灵敏度为每公斤土壤 0.25 毫克 2,4-二硝基甲苯。
