Institute of Biochemistry, Food Science, and Nutrition, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
HaEmek Medical Center, Afula, Israel.
Nutrition. 2021 Oct;90:111240. doi: 10.1016/j.nut.2021.111240. Epub 2021 Mar 24.
Broccoli is a "functional food" that contains bioactive compounds and phytochemicals that have beneficial health-promoting effects. This study aimed at investigating the effects of broccoli consumption on lipid and glucose metabolism and gut microbiota.
Male C57BL/6J mice (7-8 wk old) were fed ad libitum with a normal diet supplemented with or without 10% (w/w) broccoli florets or broccoli stalks. Oral glucose tolerance tests were performed at week 15. After 17 wk, blood and tissues were collected. Serum parameters, histology, gene and protein expression, and intestinal microbiota composition were evaluated.
Stalk supplementation led to reductions in fasting glucose levels, serum insulin, and the homeostasis model assessment-insulin resistance (HOMA-IR) index. Liver enzymes improved in both experimental groups, and broccoli florets decreased total triacylglycerols. The stalks group had elevated fatty acid oxidation-related genes and proteins (AMPK, PPARα, and CPT1). Diverse microbiota populations were observed in both broccoli groups. Broccoli stalks were found to be richer in Akkermansia muciniphila, while broccoli florets reduced Mucispirillum schaedleri abundance and increased bacterial richness.
Long-term whole broccoli supplementation decreased inflammation, improved lipid parameters and insulin sensitivity, and altered the gut microbiome in mice. Our data provide new information regarding the potential benefits of broccoli stalks in metabolic parameters.
西兰花是一种“功能性食品”，含有具有有益健康促进作用的生物活性化合物和植物化学物质。本研究旨在研究西兰花摄入对脂质和葡萄糖代谢及肠道微生物群的影响。
雄性 C57BL/6J 小鼠（7-8 周龄）自由喂食正常饮食，补充 10%（w/w）西兰花小花或西兰花茎。在第 15 周进行口服葡萄糖耐量试验。17 周后，采集血液和组织。评估血清参数、组织学、基因和蛋白质表达以及肠道微生物群落组成。
茎补充剂可降低空腹血糖水平、血清胰岛素和稳态模型评估-胰岛素抵抗（HOMA-IR）指数。两组肝脏酶均有所改善，而西兰花小花降低了总三酰甘油。茎组脂肪酸氧化相关基因和蛋白（AMPK、PPARα 和 CPT1）升高。在两组西兰花中都观察到了不同的微生物种群。发现西兰花茎中 Akkermansia muciniphila 更为丰富，而西兰花小花减少了 Mucispirillum schaedleri 的丰度并增加了细菌的丰富度。
长期全西兰花补充可降低炎症，改善脂质参数和胰岛素敏感性，并改变小鼠的肠道微生物群。我们的数据提供了关于西兰花茎在代谢参数方面潜在益处的新信息。
