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西兰花叶通过干扰血凝素和抑制病毒附着来减轻甲型流感病毒感染。

Broccoli Leaves Attenuate Influenza A Virus Infection by Interfering With Hemagglutinin and Inhibiting Viral Attachment.

作者信息

Cho Won-Kyung, Yim Nam-Hui, Lee Myong-Min, Han Chang-Hoon, Ma Jin Yeul

机构信息

Korean Medicine Application Center, Korea Institute of Oriental Medicine, Daegu, South Korea.

College of Veterinary Medicine, Jeju National University, Jeju, South Korea.

出版信息

Front Pharmacol. 2022 Jun 30;13:899181. doi: 10.3389/fphar.2022.899181. eCollection 2022.

DOI:10.3389/fphar.2022.899181
PMID:35847047
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9280179/
Abstract

Broccoli ( L. var. ) leaves are a byproduct of broccoli and could be used as a food source. The study aimed to evaluate the effect of broccoli leaves on influenza A virus (IAV) infection. We investigated the effect of ethanol extract of Broccoli leaves (EBL) on IAV infection using green fluorescent protein (GFP)-tagged Influenza A/PR/8/34 virus (PR8-GFP IAV). When EBL and PR8-GFP IAV were cotreated to RAW 264.7 cells, the fluorescence microscopy and fluorescence-activated cell sorting (FACS) analysis showed that EBL significantly reduced the levels of GFP expression by influenza viral infection dose-dependently. Immunofluorescence (IF) analysis confirmed that EBL decreased the expression of IAV proteins. EBL exhibited a strong inhibitory effect of IAV binding on the cells and moderate virucidal impact. Consistently, EBL potently suppressed the hemagglutination by IAV infection. These results indicate that EBL prevents IAV attachment the inhibition of HA upon viral infection. Finally, EBL as an HA inhibitor of IAV could be used as the natural antiviral source to protect against influenza viral infection.

摘要

西兰花（L. var.）叶是西兰花的一种副产品，可作为食物来源。本研究旨在评估西兰花叶对甲型流感病毒（IAV）感染的影响。我们使用绿色荧光蛋白（GFP）标记的甲型流感病毒A/PR/8/34（PR8-GFP IAV）研究了西兰花叶乙醇提取物（EBL）对IAV感染的影响。当EBL与PR8-GFP IAV共同处理RAW 264.7细胞时，荧光显微镜和荧光激活细胞分选（FACS）分析表明，EBL能剂量依赖性地显著降低流感病毒感染导致的GFP表达水平。免疫荧光（IF）分析证实EBL降低了IAV蛋白的表达。EBL对IAV与细胞的结合表现出强烈的抑制作用，并具有中等程度的杀病毒作用。同样，EBL能有效抑制IAV感染引起的血凝反应。这些结果表明，EBL可防止IAV附着，即抑制病毒感染时的血凝素（HA）。最后，EBL作为IAV的HA抑制剂，可作为天然抗病毒来源用于预防流感病毒感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2adf/9280179/bd4afb448ac0/fphar-13-899181-g007.jpg
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本文引用的文献

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Biomed Pharmacother. 2022 Feb;146:112581. doi: 10.1016/j.biopha.2021.112581. Epub 2021 Dec 26.
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Anti-obesity effect of sulforaphane in broccoli leaf extract on 3T3-L1 adipocytes and ob/ob mice.西兰花叶提取物中萝卜硫素对 3T3-L1 脂肪细胞和 ob/ob 小鼠的抗肥胖作用。
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In vitro virucidal activity of the theaflavin-concentrated tea extract TY-1 against influenza A virus.
茶黄素浓缩茶提取物 TY-1 抗甲型流感病毒的体外病毒灭活活性。
J Nat Med. 2022 Jan;76(1):152-160. doi: 10.1007/s11418-021-01568-0. Epub 2021 Sep 22.
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Dietary broccoli improves markers associated with glucose and lipid metabolism through modulation of gut microbiota in mice.膳食西兰花通过调节小鼠肠道微生物群改善与葡萄糖和脂质代谢相关的标志物。
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