Zandani Gil, Anavi-Cohen Sarit, Assa-Glazer Tal, Gorelick Jonathan, Nyska Abraham, Sela Noa, Bernstein Nirit, Madar Zecharia
The Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Peres Academic Center, Rehovot, Israel.
Evid Based Complement Alternat Med. 2022 Jun 26;2022:7964018. doi: 10.1155/2022/7964018. eCollection 2022.
Nonalcoholic fatty liver disease (NAFLD) is a major cause of chronic liver abnormalities and has been linked with metabolic syndrome hallmarks. Unfortunately, current treatments are limited. This work aimed to elucidate the effects of three cannabis extracts on metabolic alteration and gut microbiota composition in a mouse model of NAFLD and obesity. Male mice were fed with a high-fat diet (HFD) for 12 weeks. Following the establishment of obesity, the HFD-fed group was subdivided into HFD or HFD that was supplemented with one of three cannabis extracts (CN1, CN2, and CN6) for additional 8 weeks. Metabolic parameters together with intestinal microbiota composition were evaluated. Except for several minor changes in gene expression, no profound metabolic effect was found due to cannabis extracts addition. Nevertheless, marked changes were observed in gut microbiota diversity and composition, with CN1 and CN6 exhibiting microbial abundance patterns that are associated with more beneficial outcomes. Taken together, specific cannabis extracts' addition to an HFD results in more favorable modifications in gut microbiota. Although no marked metabolic effect was disclosed, longer treatments duration and/or higher extracts concentrations may be needed. More research is required to ascertain this conjecture and to establish the influence of various cannabis extracts on host health in general and NAFLD in particular.
非酒精性脂肪性肝病（NAFLD）是慢性肝脏异常的主要原因，并且与代谢综合征的特征相关。不幸的是，目前的治疗方法有限。这项研究旨在阐明三种大麻提取物对NAFLD和肥胖小鼠模型中代谢改变和肠道微生物群组成的影响。雄性小鼠喂食高脂饮食（HFD）12周。在肥胖症建立后，喂食HFD的组被细分为HFD组或补充三种大麻提取物（CN1、CN2和CN6）之一的HFD组，并再持续8周。评估代谢参数和肠道微生物群组成。除了基因表达的一些微小变化外，添加大麻提取物未发现明显的代谢效应。然而，在肠道微生物群多样性和组成方面观察到显著变化，CN1和CN6表现出与更有益结果相关的微生物丰度模式。总之，在HFD中添加特定的大麻提取物会导致肠道微生物群发生更有利的改变。虽然没有发现明显的代谢效应，但可能需要更长的治疗时间和/或更高的提取物浓度。需要更多的研究来证实这一推测，并确定各种大麻提取物对宿主健康尤其是对NAFLD的影响。
