Cui Fengchao, Taier Geli, Li Manli, Dai Xiaoxia, Hang Nan, Zhang Xunzhong, Wang Xiangfeng, Wang Kehua
Department of Turfgrass Science and Engineering, College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China.
Department of Breeding and Seed Science, College of Grassland Science and Technology, China Agricultural University, Beijing, 100193, China.
Hortic Res. 2021 May 1;8(1):93. doi: 10.1038/s41438-021-00519-w.
Cynodon species can be used for multiple purposes and have high economic and ecological significance. However, the genetic basis of the favorable agronomic traits of Cynodon species is poorly understood, partially due to the limited availability of genomic resources. In this study, we report a chromosome-scale genome assembly of a diploid Cynodon species, C. transvaalensis, obtained by combining Illumina and Nanopore sequencing, BioNano, and Hi-C. The assembly contains 282 scaffolds (423.42 Mb, N50 = 5.37 Mb), which cover ~93.2% of the estimated genome of C. transvaalensis (454.4 Mb). Furthermore, 90.48% of the scaffolds (~383.08 Mb) were anchored to nine pseudomolecules, of which the largest was 60.78 Mb in length. Evolutionary analysis along with transcriptome comparison provided a preliminary genomic basis for the adaptation of this species to tropical and/or subtropical climates, typically with dry summers. The genomic resources generated in this study will not only facilitate evolutionary studies of the Chloridoideae subfamily, in particular, the Cynodonteae tribe, but also facilitate functional genomic research and genetic breeding in Cynodon species for new leading turfgrass cultivars in the future.
狗牙根属植物可用于多种用途，具有很高的经济和生态意义。然而，由于基因组资源有限，人们对狗牙根属植物优良农艺性状的遗传基础了解甚少。在本研究中，我们报道了通过结合Illumina和Nanopore测序、BioNano和Hi-C技术获得的二倍体狗牙根物种——德兰士瓦狗牙根（Cynodon transvaalensis）的染色体水平基因组组装。该组装包含282个支架（约423.42 Mb，N50 = 5.37 Mb），覆盖了德兰士瓦狗牙根估计基因组（约454.4 Mb）的93.2%。此外，90.48%的支架（约383.08 Mb）被锚定到9条假染色体上，其中最长的一条长度为60.78 Mb。进化分析和转录组比较为该物种适应典型的夏季干燥的热带和/或亚热带气候提供了初步的基因组基础。本研究中产生的基因组资源不仅将促进画眉草亚科，特别是狗牙根族的进化研究，还将促进未来狗牙根属植物功能基因组研究和新的优质草坪草品种的遗传育种。
