Souza Pedro F N, Garcia-Ruiz Hernan, Carvalho Fabricio E L
Department of Plant Pathology, Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, USA.
Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Theor Exp Plant Physiol. 2019 Mar;31(1):227-248. doi: 10.1007/s40626-019-00142-0. Epub 2019 Jan 22.
Plant viruses are responsible for losses in worldwide production of numerous economically important food and fuel crops. As obligate cellular parasites with very small genomes, viruses rely on their hosts for replication, assembly, intra- and intercellular movement, and attraction of vectors for dispersal. Chloroplasts are photosynthesis and are the site of replication for several viruses. When viruses replicate in chloroplasts, photosynthesis, an essential process in plant physiology, is inhibited. The mechanisms underlying molecular and biochemical changes during compatible and incompatible plants-virus interactions, are only beginning to be elucidated, including changes in proteomic profiles induced by virus infections. In this review, we highlight the importance of proteomic studies to understand plant-virus interactions, especially emphasizing the changes in photosynthesis-related protein accumulation. We focus on: (a) chloroplast proteins that differentially accumulate during viral infection; (b) the significance with respect to chloroplast-virus interaction; and (c) alterations in plant's energetic metabolism and the subsequently the plant defense mechanisms to overcome viral infection.
植物病毒导致全球许多具有重要经济价值的粮食和燃料作物产量损失。作为基因组非常小的专性细胞寄生物，病毒依靠宿主进行复制、组装、细胞内和细胞间移动以及吸引传播媒介进行扩散。叶绿体是进行光合作用的场所，也是几种病毒的复制位点。当病毒在叶绿体中复制时，光合作用（植物生理学中的一个基本过程）会受到抑制。在亲和性和非亲和性植物 - 病毒相互作用过程中，分子和生化变化的潜在机制才刚刚开始被阐明，包括病毒感染引起的蛋白质组学图谱变化。在本综述中，我们强调蛋白质组学研究对于理解植物 - 病毒相互作用的重要性，尤其着重于光合作用相关蛋白质积累的变化。我们关注：（a）病毒感染期间差异积累的叶绿体蛋白质；（b）叶绿体 - 病毒相互作用的意义；以及（c）植物能量代谢的改变以及随后植物克服病毒感染的防御机制。
