College of Animal Science and Technology, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun 130118, China; Ministry of Education Laboratory of Animal PRODUCTION and Quality Security, Jilin Agricultural University, Changchun, China; Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, China.
College of Animal Science and Technology, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun 130118, China; Ministry of Education Laboratory of Animal PRODUCTION and Quality Security, Jilin Agricultural University, Changchun, China; Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, China.
Res Vet Sci. 2021 Jul;137:56-67. doi: 10.1016/j.rvsc.2021.04.011. Epub 2021 Apr 14.
Probiotics, including Lactobacillus rhamnosus GG ATCC53103 and Lactobacillus plantarum JL01, can improve growth performance and immunity of piglets, and relieve weaning stress-related immune disorders such as intestinal infections and inflammation. This study aimed to evaluate the ability of co-administration of the probiotics L. rhamnosus GG ATCC53103 and L. plantarum JL01 to stimulate immune responses and improve gut health during the critical weaning period in piglets. Forty-eight weaned piglets were randomly divided into four groups, and fed daily for 28 days either without, or with the two probiotics independently, or in combination. On day 28, we analyzed the cytokine and bacterial changes in intestinal mucosa and the hepatic portal vein blood metabolites of the weaned piglets. Our results showed that combined L. rhamnosus GG ATCC53103 and L. plantarum JL01 significantly increased (p < 0.05) the growth performance and expression of IL-10 and TGF-β1 mRNAs. In contrast, this treatment significantly decreased (p < 0.05) IL-1β mRNA level in the jejunum, ileum, and cecum. Furthermore, the secretion of IL-6 in the cecum, IL-1β in the jejunum, ileum, and cecum, and TNF-α in the jejunum and ileum was significantly decreased (p < 0.05). The relative abundance of Prevotella_9 and Enterococcus in ileum and cecum was significantly increased (p < 0.05). The relative abundance of Ruminococcus_1 and Ruminococcaceae_UCG-005 in cecum was significantly decreased (p < 0.05). Prevotella_9 and Enterococcus may increase the accumulation of (4Z,7Z,10Z,13Z,16Z,19Z)-4,7,10,13,16,19-docosahexaenoic acid (DHA) and tauroursodeoxycholic acid (TCDA) in portal vein blood, while Ruminococcus_1 and Ruminococcaceae_UCG-005 may decrease the accumulation of succinic and palmitic acids. These results indicate that L. rhamnosus GG ATCC53103 and L. plantarum JL01 may regulate cytokine levels by reducing the accumulation of succinic and palmitic acids and increasing the accumulation of TCDA and DHA, thereby enhancing the immunity of weaned piglets.
益生菌，包括鼠李糖乳杆菌 GG ATCC53103 和植物乳杆菌 JL01，可以提高仔猪的生长性能和免疫力，并缓解断奶应激相关的免疫紊乱，如肠道感染和炎症。本研究旨在评估同时使用益生菌鼠李糖乳杆菌 GG ATCC53103 和植物乳杆菌 JL01 是否能够在仔猪断奶的关键时期刺激免疫反应并改善肠道健康。将 48 头断奶仔猪随机分为 4 组，连续 28 天分别不添加、单独添加或同时添加这两种益生菌进行喂养。在第 28 天，分析了断奶仔猪肠道黏膜中的细胞因子和细菌变化以及肝门静脉血液中的代谢物。我们的结果表明，联合使用鼠李糖乳杆菌 GG ATCC53103 和植物乳杆菌 JL01 显著提高（p<0.05）了生长性能和 IL-10 和 TGF-β1 mRNA 的表达。相比之下，这种处理显著降低（p<0.05）了空肠、回肠和盲肠中 IL-1β mRNA 的水平。此外，回肠中 IL-6 的分泌、空肠、回肠和盲肠中 IL-1β 的分泌以及空肠和回肠中 TNF-α 的分泌均显著降低（p<0.05）。回肠和盲肠中普雷沃氏菌属 9 和肠球菌的相对丰度显著增加（p<0.05）。盲肠中瘤胃球菌 1 和毛螺菌科 UCG-005 的相对丰度显著降低（p<0.05）。普雷沃氏菌属 9 和肠球菌可能会增加门静脉血液中（4Z,7Z,10Z,13Z,16Z,19Z）-4,7,10,13,16,19-二十二碳六烯酸（DHA）和牛磺熊脱氧胆酸（TCDA）的积累，而瘤胃球菌 1 和毛螺菌科 UCG-005 可能会降低琥珀酸和棕榈酸的积累。这些结果表明，鼠李糖乳杆菌 GG ATCC53103 和植物乳杆菌 JL01 可能通过降低琥珀酸和棕榈酸的积累并增加 TCDA 和 DHA 的积累来调节细胞因子水平，从而增强断奶仔猪的免疫力。
