Obendorf D, Schwarzenbrunner U, Fischer-Colbrie R, Laslop A, Winkler H
Department of Pharmacology, University of Innsbruck, Austria.
Neuroscience. 1988 Apr;25(1):343-51. doi: 10.1016/0306-4522(88)90030-9.
A glycoprotein was isolated from detergent solubilized membranes of bovine chromaffin granules by high-performance liquid chromatography. Specific antisera raised against this glycoprotein reacted in one- and two-dimensional immunoblots with a heterogeneous component with a pI of 4.2-4.7 and Mr 100,000. The antiserum against bovine glycoprotein II cross-reacted with an analogous component in several species. The specific localization of glycoprotein II in chromaffin granules was established by density gradient centrifugation followed by immunoblotting. The antiserum, as shown by one- and two-dimensional immunoblotting, reacted with an analogous antigen in the posterior pituitary, in endocrine (anterior pituitary, parathyroid gland) and exocrine (parotid gland, pancreas) organs. In the pancreas the protein reacting with the antiserum was found in the membranes of zymogen granules. The results demonstrate for the first time that secretory vesicles of endocrine and exocrine tissues have at least one common antigen, i.e. the glycoprotein II. It seems likely that this protein is involved in a basic function common to all secretory vesicles.
通过高效液相色谱法从牛嗜铬粒蛋白颗粒的去污剂溶解膜中分离出一种糖蛋白。针对这种糖蛋白产生的特异性抗血清在一维和二维免疫印迹中与一种异质成分发生反应，该成分的等电点为4.2 - 4.7，分子量为100,000。抗牛糖蛋白II的抗血清与几个物种中的类似成分发生交叉反应。通过密度梯度离心后进行免疫印迹确定了糖蛋白II在嗜铬粒蛋白颗粒中的特异性定位。如一维和二维免疫印迹所示，该抗血清与垂体后叶、内分泌器官（垂体前叶、甲状旁腺）和外分泌器官（腮腺、胰腺）中的类似抗原发生反应。在胰腺中，与抗血清反应的蛋白质存在于酶原颗粒的膜中。结果首次证明内分泌和外分泌组织的分泌囊泡至少有一种共同抗原，即糖蛋白II。这种蛋白质似乎参与了所有分泌囊泡共有的基本功能。
