Weiler R, Steiner H J, Fischer-Colbrie R, Schmid K W, Winkler H
Department of Pharmacology, University of Innsbruck, Austria.
Histochemistry. 1991;96(5):395-9. doi: 10.1007/BF00315996.
Analysis of human and bovine serum by immunoblotting revealed the presence of the proprotein chromogranin A. By the same method chromogranin A was also found in rat, bovine and human kidney. However this organ did not contain any chromogranin A mRNA arguing against a synthesis within this organ. By immune-electron microscopy chromogranin A immunoreactivity was found in proximal tubule cells of rat kidney. Positive immunostaining was present in small vesicles within and in close proximity to the brush border and closer to the nucleus in typical lysosomal structures. These results make it likely that chromogranin A from serum reaches kidney tubule cells by glomerular filtration and is taken up into the endocytotic lysosomal pathway.
通过免疫印迹法对人血清和牛血清进行分析，结果显示存在前体蛋白嗜铬粒蛋白A。采用同样的方法，在大鼠、牛和人的肾脏中也发现了嗜铬粒蛋白A。然而，该器官中未检测到任何嗜铬粒蛋白A的信使核糖核酸，这表明该器官内不存在合成现象。通过免疫电子显微镜观察，在大鼠肾脏的近端小管细胞中发现了嗜铬粒蛋白A免疫反应性。在刷状缘内及紧邻刷状缘的小泡中以及典型溶酶体结构中靠近细胞核的位置存在阳性免疫染色。这些结果表明，血清中的嗜铬粒蛋白A可能通过肾小球滤过进入肾小管细胞，并被内吞进入溶酶体途径。
