Department of Restorative Dentistry, School of Dentistry, University of Sao Paulo (USP), Sao Paulo, SP, Brazil.
Post Graduation Program, Ibirapuera University (UNIB), Sao Paulo, SP, Brazil.
Tissue Cell. 2021 Oct;72:101536. doi: 10.1016/j.tice.2021.101536. Epub 2021 Apr 1.
Dental trauma, restorative operative procedures and/or caries lesions can expose the dental pulp. Facing this clinical condition, where the maintenance of the dentin-pulp complex vitality is imperative, is challenging in Dentistry. Dental pulp stem cells conditioned medium contains trophic factors that could help in this task. This in vivo pilot study aimed to evaluate the effects of the human dental pulp stem cells conditioned medium on the dental pulp tissue response to vital pulp therapy.
Concentrated conditioned medium was obtained by incubating characterized human dental pulp stem cells with fresh culture medium. Pulp exposures performed at the first upper molars (n = 20) of Wistar rats were directly capped with: MTA or MTA + Conditioned Medium. Four and 8 weeks later, the samples were qualitatively analyzed in histological sections (H&E).
When the conditioned medium was associated with MTA, there were a high percentage of samples presenting formation of dentin bridges and small percentage of pulp tissue with inflammatory signs in both experimental times. The conditioned medium improved the organization of the newly formed hard tissue.
The association of dental pulp stem cell conditioned medium with MTA showed beneficial effects on dentin-pulp complex regeneration and has promising potential for studies in regenerative dentistry.
牙齿外伤、修复手术和/或龋损病变都可能导致牙髓暴露。在这种情况下，维持牙本质-牙髓复合体的活力是极具挑战性的，这在牙科领域是一个难题。牙髓干细胞条件培养基中含有营养因子，可以帮助解决这个问题。本体内初步研究旨在评估人牙髓干细胞条件培养基对活髓治疗中牙髓组织反应的影响。
通过将经过鉴定的人牙髓干细胞与新鲜培养基孵育，获得浓缩的条件培养基。在 Wistar 大鼠的第一上颌磨牙（n = 20）上进行牙髓暴露，直接用 MTA 或 MTA+条件培养基覆盖。4 周和 8 周后，对样本进行组织学切片（H&E）的定性分析。
当条件培养基与 MTA 联合使用时，在两个实验时间点，有很大比例的样本显示出形成牙本质桥，而牙髓组织有炎症迹象的比例较小。条件培养基改善了新形成的硬组织的组织。
牙髓干细胞条件培养基与 MTA 的联合使用对牙本质-牙髓复合体的再生具有有益的影响，为再生牙科的研究提供了有前景的潜力。
