酸稳定性 Cu 基 MOFs 共沉淀制备可回收漆酶用于水相环境中双酚 A 的降解。
Recyclable laccase by coprecipitation with aciduric Cu-based MOFs for bisphenol A degradation in an aqueous environment.
机构信息
Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, College of Environment and Energy, South China University of Technology, Guangzhou, 510006, China.
Ministry of Education Key Laboratory of Pollution Control and Ecological Remediation for Industrial Agglomeration Area, College of Environment and Energy, South China University of Technology, Guangzhou, 510006, China; South China Institute of Collaborative Innovation, Dongguan, 523808, China.
出版信息
Colloids Surf B Biointerfaces. 2021 Aug;204:111792. doi: 10.1016/j.colsurfb.2021.111792. Epub 2021 Apr 26.
Copper-based MOF (Cu-PABA) was selected to immobilize laccase (Lac) at optimum pH because of its favorable acid resistance. Cu-PABA@Lac biocomposites were synthesized in situ by the one-step method under moderate conditions (water environment and normal temperature and pressure). Cu-PABA@Lac had great potential to maintain stability due to the protection of the Cu-PABA shell and reasonable conformational changes. In addition, Cu-PABA@Lac could be used repeatedly by centrifugation, as confirmed in the degradation experiment of bisphenol A (BPA). Because of the synergistic effect of copper ions between laccase and Cu-PABA, the K value decreased (from 0.0024 to 0.0014 mM); therefore, the affinity between laccase and guaiacol was enhanced. In conclusion, the system provides a choice for immobilized acid-resistant enzymes and a solution for environmental BPA degradation.
选用耐酸性良好的铜基金属有机框架（Cu-PABA）固定化漆酶（Lac），使其在最佳 pH 值下固定。通过一步法在温和条件下（水环境、常温常压）原位合成 Cu-PABA@Lac 生物复合材料。由于 Cu-PABA 壳的保护和合理的构象变化，Cu-PABA@Lac 具有很大的稳定性保持潜力。此外，通过离心可以重复使用 Cu-PABA@Lac，这在双酚 A（BPA）的降解实验中得到了证实。由于铜离子在漆酶和 Cu-PABA 之间的协同作用，K 值降低（从 0.0024 降至 0.0014mM），因此增强了漆酶与愈创木酚之间的亲和力。总之，该体系为固定化耐酸酶提供了选择，为环境中 BPA 的降解提供了一种解决方案。
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