College of Life Sciences, Jilin Agricultural University, Changchun, Jilin Province 130118, China.
Institute of Life Sciences, Wenzhou University, Wenzhou, Zhejiang Province 325035, China.
Res Vet Sci. 2021 Jul;137:68-76. doi: 10.1016/j.rvsc.2021.04.018. Epub 2021 Apr 22.
In this study, the effects of Goose parvovirus (GPV) infection as well as the possible role of NS1 protein on apoptosis induction in goose embryo fibroblast (GEF) cells were examined. Flow cytometry analysis and TUNEL assays revealed that GPV infection and NS1 transfection induced significant apoptosis in GEF cells compared to what was observed in mock-infected cells. Interestingly, the increase in the rate of apoptosis detected in GPV-infected GEFs was accompanied by an increased viral load in the cells. In addition, the apoptotic pathway was mediated by apoptosis-inducing factors (AIFs) and internal factors that influence the release of AIFs. The results indicated that the mitochondrial membrane potential was decreased, and AIF expression was increased in the nucleus (P < 0.01). Reactive oxygen species (ROS) increased gradually within 48 h (P < 0.001). Cathepsin D activities were also increased (P < 0.05). The results demonstrated that the AIF-mediated pathway is a new mitochondrial apoptotic pathway and that mitochondrial depolarization, ROS content, and cathepsin D activities are the key factors influencing apoptosis in GEF cells.
在这项研究中，研究了鹅细小病毒（GPV）感染以及 NS1 蛋白对鹅胚成纤维细胞（GEF）细胞凋亡诱导的可能作用。流式细胞术分析和 TUNEL 检测显示，与 mock 感染细胞相比，GPV 感染和 NS1 转染诱导 GEF 细胞发生明显的凋亡。有趣的是，在 GPV 感染的 GEF 中检测到的凋亡率增加伴随着细胞中病毒载量的增加。此外，凋亡途径是由凋亡诱导因子（AIFs）和影响 AIFs 释放的内部因素介导的。结果表明，线粒体膜电位降低，AIF 在核内表达增加（P<0.01）。48 小时内，活性氧（ROS）逐渐增加（P<0.001）。组织蛋白酶 D 活性也增加（P<0.05）。结果表明，AIF 介导的途径是一种新的线粒体凋亡途径，线粒体去极化、ROS 含量和组织蛋白酶 D 活性是影响 GEF 细胞凋亡的关键因素。
