School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, Jiangsu Province, PR China.
Food Chem. 2021 Oct 1;358:129900. doi: 10.1016/j.foodchem.2021.129900. Epub 2021 Apr 20.
In this work, a syringe needle-based integrated method was designed for the detection of biogenic amines (BAs) in raw meat samples. Based on a sequential process, the needle-based sampling, micro liquid-phase extraction and peroxidase-like catalysis were adopted for the sample collection, target analytes extraction and colorimetric analysis, respectively. The proposed method exhibited high selectivity towards BAs (the total amount of histamine, putrescine and cadaverine was utilized to present the level of BAs), where the linear range is 5-50 μM and 50-1000 μM, and the limit of detection is 1.52 μM. Specifically, the whole process could be completed in a single syringe needle. In addition, due to the minimized sampling, the change of BAs levels with time in different area of real samples (fish) can be conveniently investigated. This method has the advantages of simplicity, low cost, high sensitivity and selectivity, endowing it a promising candidate for food analysis.
在这项工作中，设计了一种基于注射器针头的集成方法，用于检测生肉样品中的生物胺 (BAs)。基于顺序过程，采用基于针头的采样、微液相萃取和过氧化物酶样催化分别用于样品采集、目标分析物提取和比色分析。该方法对 BAs 具有高选择性（总组胺、腐胺和尸胺的量用于表示 BAs 的水平），其线性范围为 5-50 μM 和 50-1000 μM，检测限为 1.52 μM。具体来说，整个过程可以在单个注射器针内完成。此外，由于采样量最小，因此可以方便地研究真实样品（鱼）不同区域中 BAs 水平随时间的变化。该方法具有简单、低成本、高灵敏度和选择性的优点，有望成为食品分析的候选方法。
