Nanoscience and Technology Interdisciplinary Program, Chulalongkorn University, Phayathai Road, Wangmai, Patumwan, Bangkok 10330, Thailand.
Metallurgy and Materials Science Research Institute, Chulalongkorn University, Phayathai Road, Wangmai, Patumwan, Bangkok 10330, Thailand.
Food Chem. 2020 Nov 1;329:127165. doi: 10.1016/j.foodchem.2020.127165. Epub 2020 May 27.
Biogenic amines are the important markers for food spoilage, thus, an on-package sensor for biogenic amine detection is crucial for food quality control. A dual detection platform including colorimetry and LDI-MS was developed for screening and quantitative determining of biogenic amines. Porous PLA film, was fabricated using calcium carbonate nanoparticles to enhance film porosity leading to increased surface area of colorimetric sensor. The color intensity significantly increases depending upon the enhanced analyte concentration with a linear range of 2.0-10.0 mg/mL for putrescine, and 0.1-6.0 mg/mL for cadaverine. On another layer, graphene oxide paper was applied as an LDI-MS substrate for sensitive quantification of biogenic amines. LOD values measured on graphene oxide coated side by LDI-MS were found to be 0.07 pM and 0.02 pM for putrescine and cadaverine, respectively. This platform was successfully applied for the detection of biogenic amines in pork samples with satisfactory results.
生物胺是食物变质的重要标志，因此，用于生物胺检测的包装内传感器对于食品质量控制至关重要。本研究开发了一种包含比色法和 LDI-MS 的双重检测平台，用于筛选和定量测定生物胺。通过使用碳酸钙纳米粒子制备了多孔 PLA 膜，以增强膜的多孔性，从而增加比色传感器的表面积。比色传感器的颜色强度随增强的分析物浓度显著增加，腐胺的线性范围为 2.0-10.0 mg/mL，尸胺的线性范围为 0.1-6.0 mg/mL。在另一层，氧化石墨烯纸被用作 LDI-MS 的基质，用于生物胺的灵敏定量。通过 LDI-MS 在氧化石墨烯涂覆侧测量的 LOD 值分别为腐胺和尸胺的 0.07 pM 和 0.02 pM。该平台成功应用于猪肉样品中生物胺的检测，结果令人满意。
