School of Science, RMIT University, Bundoora, Melbourne, Vic, 3083, Australia.
School of Science, RMIT University, Bundoora, Melbourne, Vic, 3083, Australia.
J Environ Manage. 2021 Aug 1;291:112658. doi: 10.1016/j.jenvman.2021.112658. Epub 2021 Apr 29.
Wastewaters generated by fish processing are characterised by salt concentrations similar to or greater than that of seawater together with high nutrient concentrations (e.g. organic carbon and total nitrogen) due to the presence of blood, oil, and fish tissues. Fish processing wastewater entering rivers and oceans have become a key factor leading to the pollution of receiving waters; the adequate treatment of this wastewater is, therefore, crucial to a sustainable fish industry. The present study aimed to determine whether augmentation of fish wastewater with either Marinirhabdus sp., Marinobacter hydrocarbonoclasticus or a consortium of the two halobacteria, could successfully enhance the removal of both chemical oxygen demand (COD) and total nitrogen (TN) from fish wastewater. Following 9 days of incubation, the bioaugmentation treatment resulted in a significant reduction in COD, 88%, 91%, and 92% in fish wastewater augmented with either Marinirhabdus sp., Marinobacter hydrocarbonoclasticus respectively, or a consortium of the two halobacteria compared with the control (non-bioaugmented) treatment (77% removal). In tall bioaugmentation treatments (79-88%) TN removal was also significantly greater than the control treatment (57%). After 9 days of incubation, the COD and TN in bioaugmentation reached the European Union's (EU) wastewater discharge standard (Level B, COD < 120 mg L, TN < 70 mg L). The addition of monoculture was effective in enhancing the removal of COD, while co-culture significantly improved TN removal. Results of 16S rDNA sequence analysis investigating the survival of these introduced bacteria showed that only Marinobacter hydrocarbonoclasticus was detected at the end of the treatment, constituting 36% of the total bacterial population when added alone to the wastewater. This study confirms the effectiveness of bioaugmentation in removing COD and TN in saline fish wastewater. The ability of Marinobacter hydrocarbonclasticus to enhance the treatment and dominate the bacterial community suggests the commercial potential of this organism for bioaugmentation of aquaculture wastewater without the need for further bioaugmentation.
鱼类加工产生的废水具有与海水相似或更高的盐浓度，同时由于血液、油和鱼组织的存在，还具有高营养浓度（例如有机碳和总氮）。进入河流和海洋的鱼类加工废水已成为导致受纳水体污染的关键因素；因此，对这种废水进行适当处理对于可持续的鱼类产业至关重要。本研究旨在确定是否可以通过向鱼类废水添加 Marinirhabdus sp.、Marinobacter hydrocarbonoclasticus 或这两种嗜盐菌的混合物来成功提高鱼类废水中化学需氧量 (COD) 和总氮 (TN) 的去除率。经过 9 天的孵育，与对照（未生物强化）处理（去除率为 77%）相比，用 Marinirhabdus sp.、Marinobacter hydrocarbonoclasticus 或两种嗜盐菌混合物分别强化的鱼类废水中的 COD 分别显著降低了 88%、91%和 92%。在所有生物强化处理中（79-88%），TN 的去除率也显著高于对照处理。经过 9 天的孵育，生物强化处理的 COD 和 TN 达到了欧盟（EU）废水排放标准（B 级，COD<120mg/L，TN<70mg/L）。单一培养物的添加在提高 COD 去除方面非常有效，而共培养则显著提高了 TN 的去除率。对这些引入细菌的 16S rDNA 序列分析结果表明，只有 Marinobacter hydrocarbonoclasticus 在处理结束时被检测到，当单独添加到废水中时，构成总细菌种群的 36%。本研究证实了生物强化在去除盐度鱼类废水中 COD 和 TN 的有效性。Marinobacter hydrocarbonoclasticus 增强处理并主导细菌群落的能力表明，该生物在水产养殖废水的生物强化方面具有商业潜力，而无需进一步的生物强化。
