Pharmaceutical Biology, Institute of Pharmacy, University of Greifswald, Friedrich-Ludwig-Jahn-Straße 17, 17489, Greifswald, Germany.
Pharmaceutical Microbiology, Institute of Pharmacy, University of Greifswald, Friedrich-Ludwig-Jahn-Straße 17, 17489, Greifswald, Germany.
Carbohydr Res. 2021 Jun;504:108327. doi: 10.1016/j.carres.2021.108327. Epub 2021 Apr 24.
Beta glucans are complex glucose polymers well known for their immune modulatory properties. Therefore they are used and advertised in dietary supplements. Unfortunately there is no standardized test system for quality control of such health-related foods. This approach combined wet chemical and enzyme-based quantification methods (e.g. aniline blue, Glucatell®) with a cytokine secretion assay as parameter for immune activation to resolve this problem and to establish a quality control system for β-glucan containing products and extracts. Commercially available pure β-glucans with different origin and structure were used in this study. None of the methods allowed an accurate β-glucan quantification. Most promising was the test kit K-EBHLG (Megazyme). However, cytokine secretion from whole blood was detectable under the influence of β-glucans, but there was no correlation with the quantification results using the commercially available kits. Therefore, the quality control of β-glucan containing products needs further efforts.
β-葡聚糖是一种复杂的葡萄糖聚合物，以其免疫调节特性而闻名。因此，它们被用于和宣传在膳食补充剂中。不幸的是，对于这类与健康相关的食品，没有标准化的测试系统来进行质量控制。本研究采用了湿化学和基于酶的定量方法（如苯胺蓝、Glucatell®），结合细胞因子分泌测定作为免疫激活的参数，以解决这个问题，并为含有β-葡聚糖的产品和提取物建立一个质量控制系统。本研究使用了具有不同来源和结构的市售纯β-葡聚糖。没有一种方法可以准确地定量β-葡聚糖。最有前途的是试剂盒 K-EBHLG（Megazyme）。然而，β-葡聚糖可以影响全血中细胞因子的分泌，但与市售试剂盒的定量结果没有相关性。因此，含有β-葡聚糖的产品的质量控制需要进一步努力。
