Department of Pharmaceutical Engineering, School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, People's Republic of China.
ACS Appl Mater Interfaces. 2021 May 12;13(18):21680-21692. doi: 10.1021/acsami.1c00126. Epub 2021 May 3.
Herein, a Au-Au/IrO nanocomposite with tandem enzyme-mimicking activity was innovatively synthesized, which can show outstanding glucose oxidase (GOx)-like activity and peroxidase-like activity simultaneously under neutral conditions. Moreover, a Au-Au/IrO@Cu(PABA) reactor was prepared via encapsulation of the Au-Au/IrO nanocomposite in a Cu(PABA) metal organic framework. The reactor not only exhibits excellent organic solvent stability, acid resistance, and reusability but also displays better cascade reaction catalytic efficiency (/ = 148.86 min mM) than the natural free enzyme system (GOx/HRP) (/ = 98.20 min mM) and Au-Au/IrO nanocomposite (/ = 135.24 min mM). In addition, it is found that the reactor can catalyze glucose or dissolved oxygen to produce active oxygen species (ROS) including HO, O, and O through its enzyme-mimicking activity. Finally, the novel reactor was successfully used in organic dye degradation and antibacterial application. The results show that it can effectively degrade methyl orange, methylene blue, and rhodamine B, which all can reach a degradation rate of nearly 100% after interacting with Au-Au/IrO@Cu (PABA) for 3.5 h. Furthermore, the reactor also exhibits excellent antibacterial activity, so as to achieve a complete bactericidal effect to and at a concentration of 12.5 μg mL.
本文创新性地合成了一种具有串联酶模拟活性的 Au-Au/IrO 纳米复合材料，该纳米复合材料在中性条件下可以同时表现出出色的葡萄糖氧化酶（GOx）样活性和过氧化物酶样活性。此外，通过将 Au-Au/IrO 纳米复合材料封装在 Cu(PABA) 金属有机骨架中，制备了 Au-Au/IrO@Cu(PABA) 反应器。该反应器不仅表现出优异的有机溶剂稳定性、耐酸性和可重复使用性，而且其级联反应催化效率（/ = 148.86 min mM）也优于天然游离酶体系（GOx/HRP）（/ = 98.20 min mM）和 Au-Au/IrO 纳米复合材料（/ = 135.24 min mM）。此外，研究发现该反应器可以通过其酶模拟活性，催化葡萄糖或溶解氧产生包括 HO、O 和 O 在内的活性氧物质（ROS）。最后，该新型反应器成功地应用于有机染料降解和抗菌应用中。结果表明，它可以有效地降解甲基橙、亚甲基蓝和罗丹明 B，与 Au-Au/IrO@Cu（PABA）相互作用 3.5 h 后，这些染料几乎都可以达到近 100%的降解率。此外，该反应器还表现出优异的抗菌活性，在浓度为 12.5 μg mL 时可以对 和 实现完全杀菌效果。
