Chen Mei, Li Yaxin, Huang Yu, Wang Jia, Wang Yulu, Yan Feng, Guo Weibo, Wang Xiaomeng
School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.
College of Medicine, Linyi University, Linyi 276005, China.
ACS Omega. 2025 Jun 27;10(27):29059-29073. doi: 10.1021/acsomega.5c01228. eCollection 2025 Jul 15.
Microbial evolution, combined with antibiotic overuse, has accelerated the emergence of antibiotic-resistant bacteria. These bacteria pose significant risks to human health and the ecological environment. To address this challenge, researchers are increasingly focusing on multifunctional nanostructures with multiple antimicrobial mechanisms. Herein, CuO/AuPt3 was rapidly generated by the in situ reduction of Au and Pt on the surface of CuO NPs. The decorated AuPt NPs confer CuO/AuPt3 with a high photothermal conversion efficiency of ∼56.89% within 200 s and endow the obtained material with multienzyme-mimicking activity, including peroxidase (POD)-, oxidase (OXD)-, and glutathione oxidase (GSHOx)-like activities. CuO/AuPt3 nanoparticles showed low toxicity toward L929 cells when the concentration was below 40 μg·mL. In vitro antibacterial tests demonstrated that the synergistic antibacterial mechanism resulted in effective and long-lasting antibacterial properties against both () and (). CuO/AuPt3 exhibited superior rapid bactericidal capability compared to vancomycin (VAN), and it retained good bactericidal activity even in 40% human serum. Furthermore, CuO/AuPt3 could keep silk fibroin free from contamination for at least one month at a concentration of 40 μg·mL. This study provides useful information for further research on composite materials with multienzyme-mimicking activity.
微生物进化与抗生素的过度使用相结合，加速了抗生素耐药菌的出现。这些细菌对人类健康和生态环境构成了重大风险。为应对这一挑战，研究人员越来越关注具有多种抗菌机制的多功能纳米结构。在此，通过在CuO纳米颗粒表面原位还原Au和Pt快速生成了CuO/AuPt3。修饰后的AuPt纳米颗粒使CuO/AuPt3在200秒内具有约56.89%的高光热转换效率，并赋予所得材料多种模拟酶活性，包括过氧化物酶（POD）、氧化酶（OXD）和谷胱甘肽氧化酶（GSHOx）样活性。当浓度低于40μg·mL时，CuO/AuPt3纳米颗粒对L929细胞表现出低毒性。体外抗菌试验表明，协同抗菌机制导致对（）和（）均具有有效且持久的抗菌性能。与万古霉素（VAN）相比，CuO/AuPt3表现出优异的快速杀菌能力，并且即使在40%的人血清中也能保持良好的杀菌活性。此外，在浓度为40μg·mL时，CuO/AuPt3可以使丝素蛋白至少一个月不受污染。该研究为进一步研究具有多种模拟酶活性的复合材料提供了有用信息。
