Department of Maternal and Child Health, School of Public Health, Peking University, Beijing, China.
Hospital of Health Science Center, Peking University, Beijing, China.
Pediatr Res. 2022 May;91(6):1595-1599. doi: 10.1038/s41390-021-01549-9. Epub 2021 May 2.
Metabolic disturbance of lysophosphatidylcholine (LPC) is related with dyslipidemia. Therefore, eight single-nucleotide polymorphisms (SNPs) were selected from LPC metabolic enzymes to study their associations with obesity and serum levels of lipids.
A total of 3305 children were recruited from four independent studies. Eight SNPs of LPC metabolic enzymes were selected and genotyped with the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). The multivariable linear regression model was applied to detect the associations of eight SNPs with obesity-related phenotypes and levels of lipids in each study. Meta-analyses were used to combine the results of four studies.
Only SNP rs4420638 of APOC-1 gene was associated with serum lipids even after Bonferroni correction. The rs4420638 was positively associated with TC (β = 0.15, P = 8.59 × 10) and low-density-lipoprotein-cholesterol (LDL-C, β = 0.16, P = 9.98 × 10) individually.
The study firstly revealed the association between APOC-1/rs4420638 and levels of serum lipids in Chinese children, providing evidence for susceptible gene variants of dyslipidemia.
溶血磷脂酰胆碱（LPC）的代谢紊乱与血脂异常有关。因此，从 LPC 代谢酶中选择了 8 个单核苷酸多态性（SNP），以研究它们与肥胖和血清脂质水平的关系。
从四项独立研究中招募了 3305 名儿童。选择 LPC 代谢酶的 8 个 SNP 并使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行基因分型。多元线性回归模型用于检测每个研究中 8 个 SNP 与肥胖相关表型和血脂水平的关联。使用荟萃分析合并四项研究的结果。
即使经过 Bonferroni 校正，只有载脂蛋白 C-1（APOC-1）基因的 SNP rs4420638 与血清脂质相关。rs4420638 与 TC（β=0.15，P=8.59×10）和低密度脂蛋白胆固醇（LDL-C，β=0.16，P=9.98×10）呈正相关。
本研究首次揭示了中国儿童中 APOC-1/rs4420638 与血清脂质水平之间的关联，为血脂异常的易感基因变异提供了证据。
