Zhang Shumin, Khalid A Rehman, Guo Dongmei, Zhang Jingping, Xiong Fangjie, Ren Maozhi
School of Preclinical Medicine, North Sichuan Medical College, Nanchong, China.
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Front Microbiol. 2021 Apr 16;12:596874. doi: 10.3389/fmicb.2021.596874. eCollection 2021.
, one of most famous pathogenic oomycetes, triggered the Great Irish Famine from 1845 to 1852. The target of rapamycin (TOR) is well known as a key gene in eukaryotes that controls cell growth, survival and development. However, it is unclear about its function in controlling the mycelial growth, sporulation capacity, spore germination and virulence of . In this study, key components of the TOR signaling pathway are analyzed in detail. TOR inhibitors, including rapamycin (RAP), AZD8055 (AZD), KU-0063794 (KU), and Torin1, inhibit the mycelial growth, sporulation capacity, spore germination, and virulence of with AZD showing the best inhibitory effects on Importantly, compared with a combination of RAP + KU or RAP + Torin1, the co-application of RAP and AZD show the best synergistic inhibitory effects on , resulting in the reduced dosage and increased efficacy of drugs. Transcriptome analysis supports the synergistic effects of the combination of RAP and AZD on gene expression, functions and pathways related to the TOR signaling pathway. Thus, TOR is an important target for controlling , and synergism based on the application of TOR inhibitors exhibit the potential for controlling the growth of
作为最著名的致病卵菌之一，在1845年至1852年引发了爱尔兰大饥荒。雷帕霉素靶蛋白（TOR）是真核生物中控制细胞生长、存活和发育的关键基因，广为人知。然而，其在控制[具体卵菌名称未给出]的菌丝生长、产孢能力、孢子萌发和毒力方面的功能尚不清楚。在本研究中，对TOR信号通路的关键组成部分进行了详细分析。TOR抑制剂，包括雷帕霉素（RAP）、AZD8055（AZD）、KU-0063794（KU）和Torin1，均抑制[具体卵菌名称未给出]的菌丝生长、产孢能力、孢子萌发和毒力，其中AZD对[具体卵菌名称未给出]的抑制作用最佳。重要的是，与RAP + KU或RAP + Torin1组合相比，RAP和AZD联合应用对[具体卵菌名称未给出]显示出最佳的协同抑制作用，从而降低了药物剂量并提高了疗效。转录组分析支持RAP和AZD组合对与TOR信号通路相关的基因表达、功能和途径的协同作用。因此，TOR是控制[具体卵菌名称未给出]的重要靶点，基于TOR抑制剂应用的协同作用展现出控制[具体卵菌名称未给出]生长的潜力 。
