Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, 401331 Chongqing, China.
Chongqing No.1 Secondary School, Chongqing 400044, China.
Int J Biol Macromol. 2021 Jan 1;166:1365-1376. doi: 10.1016/j.ijbiomac.2020.11.016. Epub 2020 Nov 5.
Phytophthora infestans, the pathogen of potato late blight which is a devastating disease of potatoes, causes stem and leaf rot, leading to significant economic losses. Chitosan is a naturally occurring polysaccharide with a broad spectrum of antimicrobial properties. However, the specific mechanism of chitosan on Phytophthora infestans has not been studied. In this study, we found that chitosan significantly inhibited the mycelial growth and spore germination of Phytophthora infestans in vitro, reduced the resistance of Phytophthora infestans to various adverse conditions, and it had synergistic effect with pesticides, making it a potential way to reduce the use of chemical pesticides. In addition, chitosan could induce resistance in potato pieces and leaves to Phytophthora infestans. Transcriptome analysis data showed that chitosan mainly affected cell growth of Phytophthora infestans, and most of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and Gene ontology (GO) terms revolved in metabolic processes, cell membrane structure and function and ribosome biogenesis. Differentially expressed genes (DEGs) related to adverse stress and virulence were also discussed. On the whole, this study provided new ideas for the development of chitosan as an eco-friendly preparation for controlling potato late blight.
致病疫霉，引起马铃薯晚疫病的病原体，是一种毁灭性的马铃薯疾病，导致茎和叶腐烂，造成重大的经济损失。壳聚糖是一种天然存在的多糖，具有广谱的抗菌性能。然而，壳聚糖对致病疫霉的具体作用机制尚未得到研究。在这项研究中，我们发现壳聚糖在体外显著抑制了致病疫霉的菌丝生长和孢子萌发，降低了致病疫霉对各种不利条件的抗性，并且与农药具有协同作用，因此是减少化学农药使用的一种潜在方法。此外，壳聚糖可以诱导马铃薯块茎和叶片对致病疫霉产生抗性。转录组分析数据表明，壳聚糖主要影响致病疫霉的细胞生长，京都基因与基因组百科全书（KEGG）途径和基因本体论（GO）术语的大部分都与代谢过程、细胞膜结构和功能以及核糖体生物发生有关。还讨论了与逆境和毒力相关的差异表达基因（DEGs）。总的来说，这项研究为壳聚糖作为一种防治马铃薯晚疫病的环保制剂的开发提供了新的思路。
