Liu Yuqing, Wang Xudong, Yuan Leyi, Liu Yuxiang, Shen Tong, Zhang Yunhua
Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei, China.
Front Genet. 2021 Apr 15;12:635599. doi: 10.3389/fgene.2021.635599. eCollection 2021.
Cadmium is a toxic metal widely found in workplaces and plant soil because of extensive industrialization. Wheat is an important source of food generated from plant soil. The different responses of wheat against different omic levels of cadmium have been observed and widely studied worldwide. With the development of high-throughput sequencing, micro-level biological research has extended to the microRNA level. In this study, high-cadmium-accumulating wheat cultivars (Annong9267) and low-cadmium-accumulating wheat cultivars (Qian 102032) were used as experimental models. The two cultivars were treated by Cd for 2 h to explore the microRNA profiles in root and leaf tissues through small RNA sequencing. Important small RNAs, such as tae-miR9663-5p and tae-miR6201, and potential small RNA-mediated mechanisms associated with cadmium accumulation were identified by summarizing specific microRNA profiling patterns and their respective target genes. At the wheat roots and leaves, differentially expressed small RNAs related to cadmium accumulation in different plant tissues (roots or leaves) were identified, and functional enrichment analyses on target genes of differentially expressed miRNAs in low- and high-cadmium-accumulating wheat cultivars in different plant tissues (roots or leaves) obtained some known mature miRNAs and new miRNAs. The identified miRNA will be regarded as a potential screening biomarker for low-cadmium-accumulating wheat.
镉是一种有毒金属，由于广泛的工业化，在工作场所和植物土壤中广泛存在。小麦是植物土壤产出的重要食物来源。全世界都观察到并广泛研究了小麦对不同镉含量水平的不同反应。随着高通量测序技术的发展，微观层面的生物学研究已扩展到微小RNA水平。在本研究中，高镉积累型小麦品种（安农9267）和低镉积累型小麦品种（黔102032）被用作实验模型。这两个品种用镉处理2小时，通过小RNA测序来探究根和叶组织中的微小RNA谱。通过总结特定的微小RNA谱模式及其各自的靶基因，鉴定出了重要的小RNA，如tae-miR9663-5p和tae-miR6201，以及与镉积累相关的潜在小RNA介导机制。在小麦的根和叶中，鉴定出了与不同植物组织（根或叶）中镉积累相关的差异表达小RNA，并且对不同植物组织（根或叶）中低镉积累型和高镉积累型小麦品种中差异表达微小RNA的靶基因进行功能富集分析，获得了一些已知的成熟微小RNA和新的微小RNA。所鉴定出的微小RNA将被视为低镉积累型小麦的潜在筛选生物标志物。
