Otsuka M, Meistrich M L, Brock W A
Department of Experimental Radiotherapy, University of Texas M. D. Anderson Hospital, and Tumor Institute, Houston 77030.
Radiat Res. 1988 Jul;115(1):161-75.
The distribution and kinetics of proximal tubular cells with abnormally large nuclei, which were observed in irradiated mouse kidneys before any other obvious histological effects, were investigated. Six months after the administration of 13 or 15 Gy, little histopathological change was noted, in the kidneys of C3H mice; however, proliferation of proximal tubular cells was stimulated, and some of these cells had abnormally large nuclei. The relative DNA content of these large nuclei was measured with a quantitative image analysis system. Most of the large nuclear cells had more than diploid DNA content. The labeling index of the large nuclei was higher than that of unselected proximal tubular nuclei. These cells might be hyperploid cells that are dying after having gone through an abortive mitotic division. Examination and quantitation of these abnormal nuclei should be useful in elucidating the steps involved in cell loss in the proximal tubules after irradiation and as an assay for radiation damage to the kidney.
研究了在照射后的小鼠肾脏中，在出现任何其他明显的组织学效应之前观察到的具有异常大细胞核的近端肾小管细胞的分布和动力学。给C3H小鼠给予13或15 Gy照射六个月后，其肾脏中未观察到明显的组织病理学变化；然而，近端肾小管细胞的增殖受到刺激，并且其中一些细胞具有异常大的细胞核。使用定量图像分析系统测量这些大细胞核的相对DNA含量。大多数大细胞核细胞的DNA含量超过二倍体。大细胞核的标记指数高于未选择的近端肾小管细胞核。这些细胞可能是经过失败的有丝分裂后即将死亡的超倍体细胞。对这些异常细胞核的检查和定量分析，对于阐明照射后近端肾小管细胞丢失所涉及的步骤以及作为肾脏辐射损伤的一种检测方法应该是有用的。
