Kim Na-Ye-Seul, Park Eun-Ji, Lee Seo-Hyun, Mun Kwang-Ho, Yang Ji-Young, Kim Jung-Beom
Department of Food Science and Technology, Suncheon National University, Suncheon, Jeonnam 57922 Korea.
Department of Food Science and Technology, Pukyong National University, Busan, 48513 Korea.
Food Sci Biotechnol. 2021 Mar 25;30(4):497-503. doi: 10.1007/s10068-021-00893-0. eCollection 2021 Apr.
Commercially available tunas and billfishes are generally processed as steaks, making it difficult to visually distinguish between the two. We developed and validated species-specific primers to prevent the adulteration of tunas by billfishes. Tunas and billfishes primers were designed on the cytochrome oxidase subunit I. Multiplex PCR bands obtained were 579 bp, 291 bp and 114 bp for tunas, billfishes and internal control. Sensitivity was determined to be 5 ng for tunas and billfishes. A total of 50 samples were monitored: 49 for tunas and 1 for billfish. As a result of the monitoring, the fake tunas did not show due to the agreement between product name and the raw material of the wrapping paper. Our results indicate that the species-specific primers developed in this study are suitable for differentiating tunas and billfishes. The newly developed multiplex PCR assay is a time and cost effective technique for determining the authenticity of tunas and billfishes.
市售的金枪鱼和旗鱼通常加工成鱼片，这使得在视觉上很难区分两者。我们开发并验证了物种特异性引物，以防止旗鱼掺假到金枪鱼中。金枪鱼和旗鱼的引物是根据细胞色素氧化酶亚基I设计的。获得的多重PCR条带中，金枪鱼为579 bp、291 bp和114 bp，旗鱼为291 bp和114 bp，内参为114 bp。金枪鱼和旗鱼的灵敏度测定为5 ng。共监测了50个样品：49个金枪鱼样品和1个旗鱼样品。监测结果显示，由于产品名称与包装纸原材料一致，未发现假金枪鱼。我们的结果表明，本研究中开发的物种特异性引物适用于区分金枪鱼和旗鱼。新开发的多重PCR检测方法是一种用于确定金枪鱼和旗鱼真实性的省时且经济高效的技术。
