Shen Hengyang, Huang Changzhi, Wu Jingyu, Li Jie, Hu Tao, Wang Zhenling, Zhang Hongqiang, Shao Yu, Fu Zan
Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, China.
Department of General Surgery, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, China.
Front Cell Dev Biol. 2021 Apr 15;9:656359. doi: 10.3389/fcell.2021.656359. eCollection 2021.
The complex in which scribble planar cell polarity protein () is located is one of the three main polar protein complexes that play an important role in maintaining epithelial polarity and affecting tumour growth. However, the role of in colorectal cancer (CRC) remains largely unknown. This study used date from The Cancer Genome Atlas (TCGA) and clinical samples to determine the expression of in CRC and explored its mechanism through bioinformatics analysis and and experiments. In this study, was found to be highly expressed in CRC patients, and it was often associated with malignant characteristics, such as proliferation, apoptosis, and epithelial-mesenchymal transition (EMT). Furthermore, we found that may interact with the Hippo signalling pathway and affect the phosphorylation of YAP and its distribution inside and outside of the nucleus. We concluded that increased expression of is likely to inhibit the Hippo signalling pathway by promoting YAP phosphorylation. This role of in the progression of CRC provides an important information for the treatment of CRC.
scribble平面细胞极性蛋白（）所在的复合体是在维持上皮极性和影响肿瘤生长中起重要作用的三种主要极性蛋白复合体之一。然而，其在结直肠癌（CRC）中的作用仍 largely未知。本研究使用来自癌症基因组图谱（TCGA）的数据和临床样本确定其在CRC中的表达，并通过生物信息学分析以及和实验探索其机制。在本研究中，发现其在CRC患者中高表达，且常与增殖、凋亡和上皮-间质转化（EMT）等恶性特征相关。此外，我们发现其可能与Hippo信号通路相互作用并影响YAP的磷酸化及其在细胞核内外的分布。我们得出结论，其表达增加可能通过促进YAP磷酸化来抑制Hippo信号通路。其在CRC进展中的这一作用为CRC的治疗提供了重要信息。
