Lv Qiu-Yan, Zheng He-Liang, Yang Wen-He, Liu Guo-Hua
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
Hunan Co-Innovation Center of Animal Production Safety, Changsha, China.
Front Vet Sci. 2021 Apr 15;8:649603. doi: 10.3389/fvets.2021.649603. eCollection 2021.
and are protozoan parasites that infect warm-blooded animals, and cause major economic losses in livestock industries worldwide. However, little is known about the genotypes of and in domestic ducks in China. Herein, brain samples from 588 domestic ducks from Hunan province in China were examined for the presence of and . Polymerase chain reaction (PCR) was used to detect B1 gene and gene. Forty-five DNA samples (7.7%; 95% CI: 5.5-9.9) were positive for B1 gene, and two (0.3%; 95% CI: 0-0.7) were positive for NC-5 gene. The risk factors significantly associated with infection were age and sex. The 45 samples positive for were genotyped using multi-locus PCR-RFLP analysis and only one sample was fully genotyped as ToxoDB#9 (Chinese I). These results provide new information about the epidemiology of and in ducks in Hunan province in China. The data also highlight the importance of a "One Health" approach to dealing with toxoplasmosis.
[具体寄生虫名称1]和[具体寄生虫名称2]是感染温血动物的原生动物寄生虫，在全球畜牧业中造成重大经济损失。然而，关于中国家鸭体内[具体寄生虫名称1]和[具体寄生虫名称2]的基因型，人们知之甚少。在此，对来自中国湖南省的588只家鸭的脑样本进行了[具体寄生虫名称1]和[具体寄生虫名称2]检测。采用聚合酶链反应（PCR）检测[具体寄生虫名称1]的B1基因和[具体寄生虫名称2]的NC - 5基因。45份DNA样本（7.7%；95%置信区间：5.5 - 9.9）的B1基因呈阳性，2份（0.3%；95%置信区间：0 - 0.7）的NC - 5基因呈阳性。与[具体寄生虫名称1]感染显著相关的风险因素是年龄和性别。对45份[具体寄生虫名称1]阳性样本进行多位点PCR - RFLP分析基因分型，只有1份样本被完全基因分型为ToxoDB#9（中国I型）。这些结果为中国湖南省鸭体内[具体寄生虫名称1]和[具体寄生虫名称2]的流行病学提供了新信息。这些数据还凸显了“同一健康”方法应对弓形虫病的重要性。
