1Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, China.
2State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Foodborne Pathog Dis. 2019 Aug;16(8):539-542. doi: 10.1089/fpd.2018.2605. Epub 2019 Jun 28.
is an important zoonotic parasite infecting humans and various animals with a worldwide distribution. However, limited information is available on infection in wild rats. The present study aimed to examine the prevalence and characterize the genotypes of in wild rats in two regions of China. Brain tissues were collected from 111 Edward's long-tailed rats () and 117 Bower's white-toothed rats () between November 2017 and January 2018. Genomic DNA was extracted and amplified by PCR targeting the B1 gene. B1 gene-positive samples were genotyped at 10 genetic markers (SAG1, SAG2 [5', 3'] and [alternative], SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico) using multilocus nested polymerase chain reaction/restriction fragment length polymorphism. Six (5.41%, 6/111) Edward's long-tailed rats from Chongqing Municipality were positive for B1 gene, whereas no infection was detected in Bower's white-toothed rats ( = 117) from Guangdong province. prevalence in female and male rats was 1.77% (2/113) and 3.48 (4/115), respectively. Four of the six positive DNA samples were completely genotyped at 10 genetic loci and were identified as ToxoDB#20. The present study revealed the occurrence of infection in Edward's long-tailed rats. These findings raised public health concerning about infection in wild rats. These results provide reference data for understanding the distribution of genotypes in wild rats in China.
是一种重要的人畜共患寄生虫，在全球范围内感染人类和各种动物。然而，关于野生鼠类感染的信息有限。本研究旨在检测中国两个地区野生鼠类中感染的流行情况并对其基因型进行特征分析。2017 年 11 月至 2018 年 1 月，采集了 111 只爱德华氏长尾鼠（）和 117 只鲍氏白齿鼠（）的脑组织。提取基因组 DNA，采用针对 B1 基因的 PCR 进行扩增。对 B1 基因阳性样本进行 10 个遗传标记（SAG1、SAG2[5'，3']和[替代]、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1 和 Apico）的多位点巢式聚合酶链反应/限制性片段长度多态性分析进行基因分型。来自重庆市的 6 只（5.41%，6/111）爱德华氏长尾鼠 B1 基因阳性，而来自广东省的鲍氏白齿鼠（117 只）未检测到感染。雌性和雄性鼠的感染率分别为 1.77%（2/113）和 3.48%（4/115）。6 个阳性 DNA 样本中的 4 个在 10 个遗传位点上完全基因分型，鉴定为 ToxoDB#20。本研究显示爱德华氏长尾鼠中存在感染。这些发现引起了人们对野生鼠类感染的公共卫生关注。这些结果为了解中国野生鼠类中基因型的分布提供了参考数据。
