Jin Xiaoliang, Li Yating, Yang Xing, Zheng Yadong
Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, China.
Front Vet Sci. 2021 Apr 15;8:663497. doi: 10.3389/fvets.2021.663497. eCollection 2021.
is a zoonotic tapeworm with great medical significance. In -infected mice, parasite-derived let-7-5p (emu-let-7-5p) is present in the sera, but its role remains unclear. Using qPCR, ELISA and flow cytometry, the immunomodulatory effects of emu-let-7-5p were investigated using RAW264.7 macrophages. Compared with the control, emu-let-7-5p significantly downregulated IL-1α ( < 0.05), but anti-inflammatory cytokine genes remained to be stably expressed in the treated macrophages. Moreover, significantly decreased expression of and , key components in the LPS/TLR4 signaling pathway, was also observed in the emu-let-7-5p-transfected cells ( < 0.05). Furthermore, CD40 was upregulated in these transfected cells ( < 0.05), while CD86, CD54 and CD80 remained unchanged compared that in the control. These results demonstrate a property of emu-let-7-5p in regulation of immune functions of macrophages, making it be possibly involved in the pathogen-host interplay during infection.
是一种具有重大医学意义的人畜共患绦虫。在感染的小鼠血清中存在寄生虫来源的let-7-5p（emu-let-7-5p），但其作用尚不清楚。使用qPCR、ELISA和流式细胞术，利用RAW264.7巨噬细胞研究emu-let-7-5p的免疫调节作用。与对照组相比，emu-let-7-5p显著下调IL-1α（<0.05），但抗炎细胞因子基因在处理后的巨噬细胞中仍稳定表达。此外，在emu-let-7-5p转染的细胞中也观察到LPS/TLR4信号通路中的关键成分和的表达显著降低（<0.05）。此外，这些转染细胞中CD40上调（<0.05），而与对照组相比，CD86、CD54和CD80保持不变。这些结果证明了emu-let-7-5p在调节巨噬细胞免疫功能方面的特性，使其可能参与感染期间的病原体-宿主相互作用。
