Departamento de Laboratorio Clínico, Hospital Infantil de México Federico Gómez, Mexico City, Mexico.
Subdirección de Servicios Auxiliares de Diagnóstico, Hospital Infantil de México Federico Gómez, Mexico City, Mexico.
Bol Med Hosp Infant Mex. 2021 May 3;78(3):171-180. doi: 10.24875/BMHIM.20000210.
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-making. Identification of SARS-CoV-2 infection cases is based on detection of the virus RNA by molecular tests, particularly real-time reverse transcription-polymerase chain reaction (RT-PCR). Technical and operational details specific to each center must be considered to perform the molecular diagnosis of SARS-CoV-2 in pediatric patients. The term "qualified laboratories" involves laboratories in which all users, analysts, and anyone reporting results are trained to develop and interpret results through a procedure implemented previously by an instructor. Such knowledge is essential in detecting and identifying errors during each of its phases: pre-analytical, analytical, and post-analytical, which allow the establishment of continuous improvement policies to ensure the quality of the results, but above all, the physical integrity of health workers.
2019 年冠状病毒病（COVID-19）由严重急性呼吸系统综合征 2 型冠状病毒（SARS-CoV-2）引起，目前被列为全球公共卫生紧急事件。及时识别和实施分子检测方案对于医疗决策至关重要。SARS-CoV-2 感染病例的识别基于分子检测中病毒 RNA 的检测，特别是实时逆转录聚合酶链反应（RT-PCR）。必须考虑每个中心的具体技术和操作细节，以对儿科患者进行 SARS-CoV-2 的分子诊断。术语“合格实验室”涉及所有用户、分析人员和报告结果的人员都经过培训的实验室，他们通过之前由讲师实施的程序来开发和解释结果。在其每个阶段（分析前、分析中和分析后），这种知识对于检测和识别错误至关重要，这允许建立持续改进政策，以确保结果的质量，但最重要的是，确保卫生工作者的身体健全。
