Anatomy Department and Stem Cell Unit, College of Medicine, King Saud University, Riyadh, KSA.
Pathology Department, College of Medicine, King Saud University, Riyadh, KSA.
Sci Prog. 2021 Apr-Jun;104(2):368504211011839. doi: 10.1177/00368504211011839.
The current article was designed to assess the role of chitosan nanoparticles (CNPs) in the management of hepatic injury induced by the hepatocarcinogen 2-nitropropane (2-NP). Rats were divided into three groups. The first group served as a control, the second group was injected with 2-NP, while the third group was treated with CNPs 1 h before 2-NP injection every other day for 4 weeks. The 2-NP injection upregulated serum AST and ALT activities, as well as hepatic TNF- α, IL-6, and MDA levels and the expression of vascular endothelial growth factor (VEGF) and caspase-3, whereas GSH contents and SOD activity were decreased. Immunohistochemistry investigations revealed that the hepatic protein expression of collagen I, inducible nitric oxide synthetase, proliferating cell nuclear antigen, cluster of differentiation, and p53 were upregulated. hematoxylin and eosin (H&E) and Masson's trichrome stains supported the previous parameters, and CNPs ameliorated most of the previous biochemical parameters. CNPs achieved promising results in the limitation of 2-NP hepatotoxicity.
本文旨在评估壳聚糖纳米粒子（CNPs）在管理 2-硝基丙烷（2-NP）诱导的肝损伤中的作用。大鼠被分为三组。第一组作为对照组，第二组注射 2-NP，第三组在注射 2-NP 前 1 小时每天注射 CNPs，连续 4 周。2-NP 注射上调了血清 AST 和 ALT 活性，以及肝 TNF-α、IL-6 和 MDA 水平和血管内皮生长因子（VEGF）和 caspase-3 的表达，而 GSH 含量和 SOD 活性降低。免疫组织化学研究表明，肝胶原蛋白 I、诱导型一氧化氮合酶、增殖细胞核抗原、分化簇和 p53 的蛋白表达上调。苏木精和伊红（H&E）和 Masson 三色染色支持了上述参数，CNPs 改善了大多数先前的生化参数。CNPs 在限制 2-NP 肝毒性方面取得了可喜的结果。
