Wei Xia, Qiaoli Wei, Mingzhi Liu, Chuang Liu, Qianqian Zhao, Xiaoyu Chen, Zhiwei Zhu, Xiaohuan Fang, Sa Li, Xiaolei Zhang, Weidong Hua, Jianzhi Pan, Jianhui Tian, Junjie Li
College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
College of Life Science and Technology, Southwest Minzu University, Chengdu, China.
Anim Biotechnol. 2022 Dec;33(7):1510-1518. doi: 10.1080/10495398.2021.1914068. Epub 2021 May 3.
Utilization of female livestock can be optimized by application of Fixed-Time Artificial Insemination (FTAI), which plays an important role in large-scale livestock production. However, molecular mechanism of FTAI affecting reproductive performance remains unclear. To investigate the effects of FTAI on corpus luteum in 16 and 25 days of pregnancy gilts, 12 pregnancy gilts were selected from Altrenogest + PMSG + GnRH (APG) group and control group. The number of left and right CL in APG group were significantly higher than control ( < 0.05). Furthermore, result showed the number of differentially expressed genes between 16APG group and 16 C group was obviously larger than that between 25APG and 25 C group (2394 vs. 1476). Up regulated genes in APG were mainly associated with cytokine production and secretion, cell communication and transport (day 16) and angiogenesis, cell cycle and cell-cell signaling (day 25). The expression of differentially expressed genes (RPL10, CYP17A1, DCN, IL18, LDLR and PRLR) in luteal tissue were verified by real-time PCR. In summary, APG program significantly improve reproductive efficiency of gilts through up regulation of cytokine production/secretion, cell communication and transport in Day 16 pregnancy and angiogenesis, cell cycle and cell-cell signaling at Day 25 pregnancy in porcine.
通过应用定时人工授精（FTAI）可以优化雌性家畜的利用，这在大规模家畜生产中起着重要作用。然而，FTAI影响繁殖性能的分子机制仍不清楚。为了研究FTAI对妊娠16天和25天的后备母猪黄体的影响，从烯丙孕素+孕马血清促性腺激素+促性腺激素释放激素（APG）组和对照组中选取12头妊娠后备母猪。APG组左右两侧黄体的数量显著高于对照组（P<0.05）。此外，结果显示16APG组和16C组之间差异表达基因的数量明显多于25APG组和25C组（2394对1476）。APG组上调的基因主要与细胞因子的产生和分泌、细胞通讯和转运（第16天）以及血管生成、细胞周期和细胞间信号传导（第25天）有关。通过实时PCR验证了黄体组织中差异表达基因（RPL10、CYP17A1、DCN、IL18、LDLR和PRLR）的表达。总之，APG程序通过上调妊娠第16天的细胞因子产生/分泌、细胞通讯和转运以及妊娠第25天的血管生成、细胞周期和细胞间信号传导，显著提高了后备母猪的繁殖效率。
