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转录因子IRF6对KIF20A的抑制作用影响肾透明细胞癌的进展。

Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma.

作者信息

Ma Xinwei, Wang Xiaoqi, Dong Qian, Pang Hongquan, Xu Jianming, Shen Junkang, Zhu Jianbing

机构信息

Department of Radiology, The Second Affiliated Hospital of Soochow University, No.1055 Sanxiang Road, Suzhou, 215004, Jiangsu, China.

Department of Radiology, Suzhou Science and Technology Town Hospital, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, No.1 Lijiang Road, high Tech Zone, Suzhou, 215153, Jiangsu, China.

出版信息

Cancer Cell Int. 2021 May 3;21(1):246. doi: 10.1186/s12935-021-01879-y.

DOI:10.1186/s12935-021-01879-y
PMID:33941190
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8091794/
Abstract

BACKGROUND

Renal clear cell carcinoma (ccRCC) is one of the most common malignant tumors, whose incidence is increasing year by year. IRF6 plays an important role in the occurrence of tumors, although there is yet no report on its expression in ccRCC.

METHODS

The expression of IRF6 and KIF20A in ccRCC was predicted by GEPIA and HAP databases. In addition, GEPIA database predicted the relationship between IRF6 and KIF20A expressions and the pathological staging, overall survival, and disease-free survival of ccRCC. The possible binding sites of IRF6 and KIF20A promoters were predicted by JASPAR database and verified by luciferase and ChIP assays. The specific effects of IRF6 on ccRCC cell proliferation, invasion and apoptosis were subsequently examined at both cellular level and animal level.

RESULTS

The database predicted down-regulated IRF6 expression in renal carcinoma tissues and its correlation with poor prognosis. IRF6 overexpression inhibited cRCC cell proliferation, invasion and migration. In addition, up-regulated KIF20A expression in renal carcinoma tissues and its association with prognosis were also predicted. Interference with KIF20A inhibited the proliferation, invasion, and migration of ccRCC cells. Finally, we confirmed that KIF20A is a functional target of IRF6 and can partially reverse the effects of IRF6 on the proliferation, invasion and migration of ccRCC cells.

CONCLUSION

Inhibition of KIF20A by transcription factor IRF6 affects cell proliferation, invasion and migration in renal clear cell carcinoma.

摘要

背景

肾透明细胞癌（ccRCC）是最常见的恶性肿瘤之一，其发病率逐年上升。IRF6在肿瘤发生中起重要作用，尽管尚无关于其在ccRCC中表达的报道。

方法

通过GEPIA和HAP数据库预测IRF6和KIF20A在ccRCC中的表达。此外，GEPIA数据库预测了IRF6和KIF20A表达与ccRCC的病理分期、总生存期和无病生存期之间的关系。通过JASPAR数据库预测IRF6和KIF20A启动子的可能结合位点，并通过荧光素酶和染色质免疫沉淀试验进行验证。随后在细胞水平和动物水平上研究IRF6对ccRCC细胞增殖、侵袭和凋亡的具体影响。

结果

数据库预测肾癌组织中IRF6表达下调及其与预后不良的相关性。IRF6过表达抑制cRCC细胞的增殖、侵袭和迁移。此外，还预测了肾癌组织中KIF20A表达上调及其与预后的关系。干扰KIF20A可抑制ccRCC细胞的增殖、侵袭和迁移。最后，我们证实KIF20A是IRF6的功能靶点，并且可以部分逆转IRF6对ccRCC细胞增殖、侵袭和迁移的影响。

结论

转录因子IRF6对KIF20A的抑制作用影响肾透明细胞癌的细胞增殖、侵袭和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/174f2d657594/12935_2021_1879_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/f10ae026f851/12935_2021_1879_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/ef74b6aedc52/12935_2021_1879_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/5000109419b3/12935_2021_1879_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/5e72bb05fd62/12935_2021_1879_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/2b57c5421fde/12935_2021_1879_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/8dc24fda5448/12935_2021_1879_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/9b9df98aaa82/12935_2021_1879_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/4fe7d2d9b243/12935_2021_1879_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/174f2d657594/12935_2021_1879_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/f10ae026f851/12935_2021_1879_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/ef74b6aedc52/12935_2021_1879_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/5000109419b3/12935_2021_1879_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/5e72bb05fd62/12935_2021_1879_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/2b57c5421fde/12935_2021_1879_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/8dc24fda5448/12935_2021_1879_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/9b9df98aaa82/12935_2021_1879_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/4fe7d2d9b243/12935_2021_1879_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/835d/8091794/174f2d657594/12935_2021_1879_Fig9_HTML.jpg

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