Department of Oncology, Cancer Center, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences, Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou, China.
Research and Experimental Center, Meizhou People's Hospital (Huangtang Hospital), Meizhou Academy of Medical Sciences, Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou, China.
J Int Med Res. 2021 May;49(5):3000605211012570. doi: 10.1177/03000605211012570.
Colorectal cancer (CRC) is one of the most common and lethal malignancies. The identification of precise and noninvasive biomarkers is urgently needed to aid the early diagnosis and clinical management of CRC.
A total of 112 patients with CRC and 115 healthy control subjects were included in this study. Serum levels of matrix metalloproteinase (MMP)-7, MMP-9, MMP-11, tissue inhibitor of metalloproteinase (TIMP)-1, and TIMP-2 were analyzed by enzyme-linked immunosorbent assay, and carcinoembryonic antigen (CEA) and carbohydrate antigen (CA)19-9 levels were measured using an automatic immunoassay analyzer.
MMP-7, MMP-9, MMP-11, TIMP-1, TIMP-2, CEA, and CA19-9 levels were all significantly higher in CRC patients compared with healthy controls. MMP-7, TIMP-1, and CEA levels were also closely related to clinicopathologic features in patients with CRC. The combination of serum CEA, MMP-7, and TIMP-1 significantly improved the diagnostic value compared with any single marker (area under the curve 0.858-0.890). Furthermore, a combined detection model including MMP-7, TIMP-1, and CEA improved both the specificity and sensitivity for detecting CRC.
The results showed that combined detection of CEA, MMP-7, and TIMP-1 in serum could provide a specific and sensitive biomarker for the diagnosis of CRC.
结直肠癌（CRC）是最常见和最致命的恶性肿瘤之一。迫切需要确定精确和非侵入性的生物标志物，以辅助 CRC 的早期诊断和临床管理。
本研究共纳入 112 例 CRC 患者和 115 例健康对照者。采用酶联免疫吸附试验分析血清基质金属蛋白酶（MMP）-7、MMP-9、MMP-11、金属蛋白酶组织抑制剂（TIMP）-1 和 TIMP-2 水平，采用自动免疫分析仪检测癌胚抗原（CEA）和糖类抗原（CA）19-9 水平。
CRC 患者的 MMP-7、MMP-9、MMP-11、TIMP-1、TIMP-2、CEA 和 CA19-9 水平均明显高于健康对照组。MMP-7、TIMP-1 和 CEA 水平也与 CRC 患者的临床病理特征密切相关。与任何单一标志物相比，血清 CEA、MMP-7 和 TIMP-1 的联合检测显著提高了诊断价值（曲线下面积 0.858-0.890）。此外，包括 MMP-7、TIMP-1 和 CEA 的联合检测模型提高了检测 CRC 的特异性和敏感性。
结果表明，联合检测血清 CEA、MMP-7 和 TIMP-1 可提供 CRC 诊断的特异性和敏感性生物标志物。
