Department of Biosciences and Territory, University of Molise, Contrada Fonte Lappone, Pesche (IS), 86090, Italy.
Institute for Sustainable Plant Protection (IPSP), Turin Unit, National Research Council, Viale P.A. Mattioli 25, Turin, 10125, Italy.
Environ Microbiol Rep. 2021 Oct;13(5):591-599. doi: 10.1111/1758-2229.12956. Epub 2021 May 4.
Molise region (Central-Southern Italy) is one of the Italian richest areas of truffles and contributes significantly to the national production of the precious Tuber magnatum. Nevertheless, Molise truffle has received little scientific attention. Accordingly, in the present study, two T. magnatum populations collected in two different sites of Molise region were characterised from a morphological, genetic and microbiological point of view. A considerable variability between and within the two analysed groups emerged, suggesting an interesting heterogeneity of Molise white truffle populations. Ascocarps of the two groups significantly differed in size and maturation degree, although no linear correlation between weight and maturity was found. Genetic investigations focused on the Sequence-Characterised Amplified Region SCAR A21-inf. Three haplotypes, randomly distributed within the two truffle groups regardless of their collection sites, were detected. The 16S rRNA gene amplicon high-throughput sequencing provided an overview of the composition of the ascocarp-associated bacterial communities. A predominance of α-Proteobacteria was observed, with Bradyrhizobium among the main genera. However, some truffles showed unusual microbial profiles, with Pedobacter, Polaromonas and other bacterial genera as dominant taxa.
莫利塞地区（意大利中南部）是意大利产松露最丰富的地区之一，对国家珍贵的白松露（Tuber magnatum）产量贡献巨大。然而，莫利塞松露很少受到科学界的关注。因此，在本研究中，从形态学、遗传学和微生物学的角度对莫利塞地区两个不同地点采集的两个白松露种群进行了特征描述。两个分析群体之间和内部都出现了相当大的变异，表明莫利塞白松露种群具有有趣的异质性。两个群体的子实体在大小和成熟度上有显著差异，尽管在重量和成熟度之间没有发现线性相关性。遗传研究集中在序列特征扩增区 SCAR A21-inf 上。在两个松露群体中，无论其采集地点如何，都检测到三个随机分布的单倍型。16S rRNA 基因扩增子高通量测序提供了对附属子实体细菌群落组成的概述。观察到α-变形菌的优势，其中根瘤菌是主要属之一。然而，一些松露表现出不寻常的微生物特征，Pedobacter、Polaromonas 和其他细菌属为优势类群。
